Jin Ge, Tang Qiang, Ma Jiaheng, Liu Xiang, Zhou Bingqian, Sun Yue, Pang Xiaoqi, Guo Zixuan, Xie Runxiang, Liu Tianyu, Wang Bangmao, Cao Hailong
Department of Gastroenterology and Hepatology, General Hospital, Tianjin Medical University, Tianjin, China.
Tianjin Institute of Digestive Diseases, Tianjin Key Laboratory of Digestive Diseases, Tianjin, China.
mSystems. 2021 Mar 16;6(2):e01337-20. doi: 10.1128/mSystems.01337-20.
Early life events can lead to multiple diseases in adulthood. Previous studies suggested that polysorbate 80 (P80) as a widely used emulsifier in pharmaceutical formulations and food industries could impair the intestinal barrier. However, whether maternal P80 (MP80) exposure could affect the long-term health of offspring remains unknown. In this study, we found that maternal P80 intake could retard intestinal development, disrupt the intestinal barrier, and cause low-grade intestinal inflammation in 3-week-old offspring. 16S rRNA sequencing and correlation analysis revealed that , , and , which positively correlated with intestinal proliferation and differentiation, were decreased in the maternal P80 group. Interestingly, the increase in some harmful bacteria, including , , and , persisted from the weaning period to adulthood (3 to 8 weeks). Furthermore, a fecal microbiota transplantation assay showed that the mice gavaged with feces from 3-week-old offspring of the MP80 group presented more severe intestinal inflammation and barrier disruption than the mice that received feces from the offspring of the control group. Finally, maternal P80 intake remarkably aggravated the structural disorder of intestinal crypt, increased proinflammatory factors, and exacerbated dextran sulfate sodium (DSS)-induced colitis in adulthood. Conclusively, maternal P80 intake could induce gut dysbiosis and promote colitis susceptibility in adulthood. This study provides new insights into the prevention of inflammatory bowel disease (IBD). The main findings of this research showed that maternal P80 intake could disrupt the intestinal barrier, induce gut dysbiosis, and promote colitis susceptibility in adulthood. This study will enhance understanding of the prevention of IBD.
早期生活事件可导致成年后患上多种疾病。先前的研究表明，聚山梨醇酯80（P80）作为制药配方和食品工业中广泛使用的乳化剂，可能会损害肠道屏障。然而，母体暴露于P80（MP80）是否会影响后代的长期健康仍不清楚。在本研究中，我们发现母体摄入P80会延缓3周龄后代的肠道发育、破坏肠道屏障并导致低度肠道炎症。16S rRNA测序和相关性分析显示，与肠道增殖和分化呈正相关的[具体细菌名称1]、[具体细菌名称2]和[具体细菌名称3]在母体P80组中减少。有趣的是，包括[具体有害细菌名称1]、[具体有害细菌名称2]和[具体有害细菌名称3]在内的一些有害细菌的增加从断奶期持续到成年期（3至8周）。此外，粪便微生物群移植试验表明，用MP80组3周龄后代的粪便灌胃的小鼠比接受对照组后代粪便的小鼠表现出更严重的肠道炎症和屏障破坏。最后，母体摄入P80显著加剧了成年期肠道隐窝的结构紊乱，增加了促炎因子，并加重了葡聚糖硫酸钠（DSS）诱导的结肠炎。总之，母体摄入P80可导致肠道微生物群失调，并增加成年期患结肠炎的易感性。本研究为炎症性肠病（IBD）的预防提供了新的见解。本研究的主要发现表明，母体摄入P80会破坏肠道屏障，诱导肠道微生物群失调，并增加成年期患结肠炎的易感性。本研究将增进对IBD预防的理解。
