State Key Laboratory of Bioreactor Engineering, Department of Food Science and Engineering, School of Biotechnology, East China University of Science and Technology, P. O. Box 283130 # Meilong Rd, Shanghai, 200237, People's Republic of China.
Department of Biotechnology, Faculty of Life Science, Kim Hyong Jik University of Education, Pyongyang, Democratic People's Republic of Korea.
Eur J Nutr. 2024 Apr;63(3):697-711. doi: 10.1007/s00394-023-03291-w. Epub 2023 Dec 26.
Probiotics have been reported to effectively alleviate hyperuricemia and regulate the gut microbiota. The aim of this work was to study the in vivo anti-hyperuricemic properties and the mechanism of a novel strain, Lactiplantibacillus plantarum X7022.
Purine content and mRNA expression of purine assimilation related enzymes were determined by HPLC and qPCR, respectively. Hyperuricemic mice were induced by potassium oxonate and hypoxanthine. Uric acid (UA), blood urea nitrogen, creatinine and renal inflammation were examined by kits. The expression of renal UA transporters was subjected to western blotting. Kidney tissues were sectioned for histological analysis. The fecal short-chain fatty acids (SCFAs) were determined by HPLC, and gut microbiota was investigated using the 16S rDNA metagenomic sequencing.
L. plantarum X7022 possesses a complete purine assimilation pathway and can exhaust xanthine, guanine, and adenine by 82.1%, 33.1%, and 12.6%, respectively. The strain exhibited gastrointestinal viability as 44% at the dose of 10 CFU/mL in mice. After four-week administration of the strain, a significant decrease of 35.5% in the serum UA level in hyperuricemic mice was achieved. The diminished contents of fecal propionate and butyrate were dramatically boosted. The treatment also alleviated renal inflammation and restored renal damage. The above physiological changes may due to the inhibited xanthine oxidase (XO) activity, as well as the expressional regulation of UA transporters (GLUT9, URAT1 and OAT1) to the normal level. Notably, gut microbiota dysbiosis in hyperuricemic mice was improved with the inflammation and hyperuricemia related flora depressed, and SCFAs production related flora promoted.
The strain is a promising probiotic strain for ameliorating hyperuricemia.
益生菌已被报道能有效缓解高尿酸血症并调节肠道微生物群。本工作旨在研究新型植物乳杆菌 X7022 的体内抗高尿酸血症特性和作用机制。
采用 HPLC 和 qPCR 分别测定嘌呤含量和嘌呤同化相关酶的 mRNA 表达。用氧嗪酸钾和次黄嘌呤诱导高尿酸血症小鼠。试剂盒检测尿酸（UA）、血尿素氮、肌酐和肾脏炎症。Western 印迹法检测肾脏 UA 转运蛋白的表达。对肾脏组织进行切片进行组织学分析。采用 HPLC 测定粪便短链脂肪酸（SCFAs），采用 16S rDNA 宏基因组测序研究肠道微生物群。
植物乳杆菌 X7022 具有完整的嘌呤同化途径，可分别消耗 82.1%、33.1%和 12.6%的黄嘌呤、鸟嘌呤和腺嘌呤。该菌株在小鼠中的胃肠道存活率为 44%，剂量为 10 CFU/mL。经过四周的菌株给药后，高尿酸血症小鼠血清 UA 水平显著降低 35.5%。粪便丙酸和丁酸含量明显增加。治疗还可减轻肾脏炎症并恢复肾脏损伤。上述生理变化可能是由于黄嘌呤氧化酶（XO）活性受到抑制，以及 UA 转运蛋白（GLUT9、URAT1 和 OAT1）的表达调节至正常水平所致。值得注意的是，高尿酸血症小鼠的肠道微生物群失调得到改善，炎症和高尿酸血症相关菌群受到抑制，而 SCFAs 产生相关菌群得到促进。
该菌株是一种有前途的改善高尿酸血症的益生菌菌株。
