Medical University of Lodz, Department of Biochemistry, Faculty of Medicine, Lodz, Poland.
Lodz University of Technology, Institute of Molecular and Industrial Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz, Poland.
J Physiol Pharmacol. 2020 Dec;71(6). doi: 10.26402/jpp.2020.6.07. Epub 2021 Mar 13.
In the present study we investigated the anti-inflammatory activity of Japanese quince leaf polyphenol-rich extract (JQLPE) in lipopolysaccharide (LPS)-activated murine macrophages (RAW 264.7). The Q-PCR analysis revealed that JQLPE decreased Nfkb1, Ptgs2, and Il1b expression at the mRNA level by 80%, 50% and 48%, respectively. Similarly, JQLPE significantly attenuated expression of inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), interleukin-1beta (IL-1β), IL-6 and tumor necrosis factor alpha (TNF-α) (by 60%, 50%, 67%, 37% and 36%, respectively) at the protein level and nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7. Western blot also showed that the expression of nuclear factor kappaB (NF-κB) p65 and p-NF-κB p65 was down-regulated after JQLPE treatment. These results provide the first evidence that JQLPE decreases the expression of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α), inflammatory mediators (COX-2, iNOS) and both NF-κB p65 and p-NF-κB p65 in LPS-stimulated RAW264.7 cells, which may suggest its anti-inflammatory activity.
在本研究中，我们研究了日本榅桲叶多酚丰富提取物（JQLPE）在脂多糖（LPS）激活的小鼠巨噬细胞（RAW 264.7）中的抗炎活性。Q-PCR 分析显示，JQLPE 分别使 Nfkb1、Ptgs2 和 Il1b 的表达在 mRNA 水平降低了 80%、50%和 48%。同样，JQLPE 显著减弱了诱导型一氧化氮合酶（iNOS）、环氧合酶 2（COX-2）、白细胞介素-1β（IL-1β）、白细胞介素-6 和肿瘤坏死因子-α（TNF-α）的表达（分别降低了 60%、50%、67%、37%和 36%），并降低了脂多糖（LPS）刺激的 RAW264.7 中的一氧化氮（NO）产生。Western blot 还显示，JQLPE 处理后核因子 kappaB（NF-κB）p65 和 p-NF-κB p65 的表达下调。这些结果首次提供了证据，证明 JQLPE 降低了 LPS 刺激的 RAW264.7 细胞中促炎细胞因子（IL-1β、IL-6、TNF-α）、炎症介质（COX-2、iNOS）以及 NF-κB p65 和 p-NF-κB p65 的表达，这可能表明其具有抗炎活性。
