School of Life Sciences and Food Engineering, Hebei University of Engineering, Handan, China.
Anim Sci J. 2021 Jan-Dec;92(1):e13541. doi: 10.1111/asj.13541.
Toll-like receptors (TLRs) participate in regulation of adaptive immune responses, and lymph nodes play key roles in the initiation of immune responses. There is a tolerance to the allogenic fetus during pregnancy, but it is unclear that expression of TLR signaling is in ovine lymph node during early pregnancy. In this study, lymph nodes were sampled from day 16 of nonpregnant ewes and days 13, 16, and 25 of pregnant ewes, and the expressions of TLR family (TLR2, TLR3, TLR4, TLR5 and TLR9), adaptor proteins, including myeloid differentiation primary-response protein 88 (MyD88), tumor necrosis factor receptor associated factor 6 (TRAF6), and interleukin-1-receptor-associated kinase 1 (IRAK1), were analyzed through real-time quantitative polymerase chain reaction, Western blot, and immunohistochemistry analysis. The results showed that mRNA and protein levels of TLR2, TLR3, TLR4, TRAF6, and MyD88 were upregulated in the maternal lymph node, but TLR5, TLR9, and IRAK1 were downregulated during early pregnancy. In addition, MyD88 protein was located in the subcapsular sinus and lymph sinuses. Therefore, it is suggested that early pregnancy induces changes in TLR signaling in maternal lymph node, which may be involved in regulation of maternal immune responses in sheep.
Toll 样受体（TLRs）参与调节适应性免疫反应，淋巴结在免疫反应的启动中起着关键作用。怀孕期间对同种异体胎儿有耐受性，但尚不清楚 TLR 信号在绵羊淋巴结中的早期妊娠表达情况。在这项研究中，从非妊娠母羊的第 16 天和妊娠母羊的第 13、16 和 25 天采集淋巴结，并通过实时定量聚合酶链反应、Western blot 和免疫组织化学分析分析 TLR 家族（TLR2、TLR3、TLR4、TLR5 和 TLR9）、衔接蛋白（包括髓样分化初级反应蛋白 88（MyD88）、肿瘤坏死因子受体相关因子 6（TRAF6）和白细胞介素-1 受体相关激酶 1（IRAK1）的表达。结果表明，母性淋巴结中 TLR2、TLR3、TLR4、TRAF6 和 MyD88 的 mRNA 和蛋白水平上调，但 TLR5、TLR9 和 IRAK1 在早孕时下调。此外，MyD88 蛋白位于被膜下窦和淋巴窦中。因此，提示早孕诱导母性淋巴结 TLR 信号的变化，这可能参与绵羊母体免疫反应的调节。
