College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan, 056038, China.
College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan, 056038, China.
Reprod Biol. 2020 Dec;20(4):547-554. doi: 10.1016/j.repbio.2020.10.003. Epub 2020 Nov 4.
Toll-like receptors (TLRs) participates in regulation of the maternal immune tolerance during pregnancy, and the thymus is critical for the adaptive immune system. This study hypothesized whether early pregnancy affected the expression of toll-like receptor pathway in the thymus of ewes. In this study, expression of TLRs, tumor necrosis factor receptor associated factor 6 (TRAF6), interleukin 1 receptor associated kinase 1 (IRAK1) and myeloid differentiation primary response gene 88 (MyD88) was detected in maternal thymus during early pregnancy in sheep. Ovine thymuses were collected on day 16 of the estrous cycle, and days 13, 16 and 25 of pregnancy, and expression of TLR members was analyzed by real-time quantitative PCR, western blot and immunohistochemistry analysis. The results revealed that there were decreases in the expression of the mRNA and proteins of TLR2, IRAK1, TRAF6 and MyD88, but increase in TLR5 mRNA and protein. Furthermore, expression of TLR3 and TLR4 proteins peaked at days 13 and 16 of gestation, and MyD88 protein was located in the epithelial reticular cells and thymic corpuscles. In summary, TLR signaling is implicated in regulation of maternal thymic immune, which may be via downregulation of TLR2, IRAK1, TRAF6 and MyD88 during early pregnancy in sheep.
Toll 样受体（TLRs）参与妊娠期间母体免疫耐受的调节，而胸腺对于适应性免疫系统至关重要。本研究假设早期妊娠是否会影响母羊胸腺中 Toll 样受体途径的表达。在这项研究中，检测了绵羊发情周期第 16 天和妊娠第 13、16 和 25 天母羊胸腺中 TLRs、肿瘤坏死因子受体相关因子 6（TRAF6）、白细胞介素 1 受体相关激酶 1（IRAK1）和髓样分化原初反应基因 88（MyD88）的表达。通过实时定量 PCR、western blot 和免疫组织化学分析检测 TLR 成员的表达。结果表明，TLR2、IRAK1、TRAF6 和 MyD88 的 mRNA 和蛋白表达减少，但 TLR5 的 mRNA 和蛋白表达增加。此外，TLR3 和 TLR4 蛋白的表达在妊娠第 13 和 16 天达到峰值，MyD88 蛋白位于上皮网状细胞和胸腺小体中。总之，TLR 信号通路参与调节母体胸腺免疫，这可能是通过在绵羊妊娠早期下调 TLR2、IRAK1、TRAF6 和 MyD88 来实现的。
