Roos M S, Apfel R E, Wardlaw S C
Applied Mechanics, Yale University, New Haven, Connecticut 06520.
J Acoust Soc Am. 1988 Apr;83(4):1639-44. doi: 10.1121/1.395918.
A technique for simultaneously measuring the scattering amplitude of individual particles at two angles is applied to human red blood cells. Using a Rayleigh scattering model, the density and compressibility of the cells may be determined given a priori knowledge of their volume. A calibration method relying on measurements of the bulk properties of particle suspensions is described. Red cell properties in hypotonic and hypertonic hosts are compared with a homogeneous mixture model, and a linear relation between hemoglobin content and scattering amplitude at a 90 deg scattering angle is established.
一种同时测量单个粒子在两个角度散射振幅的技术被应用于人类红细胞。利用瑞利散射模型，在已知细胞体积的先验知识的情况下，可以确定细胞的密度和可压缩性。描述了一种基于测量粒子悬浮液整体性质的校准方法。将低渗和高渗环境中红细胞的性质与均匀混合模型进行比较，并建立了血红蛋白含量与90度散射角处散射振幅之间的线性关系。
