Frank K H, Kessler M, Appelbaum K, Albrecht H P, Mauch E D
Institut für Physiologie und Kardiologie, Erlangen, Federal Republic of Germany.
Phys Med Biol. 1989 Dec;34(12):1901-16. doi: 10.1088/0031-9155/34/12/012.
Remission spectroscopy in living tissues can only be performed because scattering processes induce a pronounced amount of backscattered light. New types of scattering chambers were constructed in order to investigate the angular distribution of light intensities caused by scattering events typical for living tissues. Different solutions and suspensions containing microspheres, haemoglobin molecules, erythrocytes and liver homogenate were used in order to gain basic information applicable to remission spectrophotometry in tissue. Reflection spectroscopy in biological tissues is only possible because of the scattering properties of the material under observation. Light which penetrates the sample is remitted from the tissue. The remission is caused by the scattering of the light by different biological structures and particles. This scattered light gives information about the optical properties of the particles and structures in an integral form. A scattering chamber for small sample volumes has been developed for measurements of the optical properties of various particles in suspension. Measurements of scattering diagrams from microspheres with an average diameter of 7 microns, artificial particles, haemoglobin solutions, mixtures of microspheres and haemoglobin as well as erythrocytes and mitochondria have been performed. The different optical behaviour of the measured signals is qualitatively discussed.
活体组织中的漫反射光谱分析之所以能够进行，仅仅是因为散射过程会产生大量的后向散射光。为了研究由活体组织典型的散射事件所引起的光强角分布，人们构建了新型的散射室。使用了含有微球、血红蛋白分子、红细胞和肝脏匀浆的不同溶液和悬浮液，以便获取适用于组织漫反射分光光度法的基础信息。生物组织中的反射光谱分析之所以可行，仅仅是因为被观察材料的散射特性。穿透样品的光从组织中反射回来。这种漫反射是由光被不同的生物结构和颗粒散射所引起的。这种散射光以积分形式给出了有关颗粒和结构光学特性的信息。已经开发出一种用于小样本体积的散射室，用于测量悬浮液中各种颗粒的光学特性。已经对平均直径为7微米的微球、人工颗粒、血红蛋白溶液、微球与血红蛋白的混合物以及红细胞和线粒体的散射图谱进行了测量。对所测信号的不同光学行为进行了定性讨论。
