Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Kaohsiung J Med Sci. 2021 Jul;37(7):547-561. doi: 10.1002/kjm2.12378. Epub 2021 Mar 17.
Circular RNAs (circRNAs) appear to be significant modulators in various physiological processes. Recently, it is found that circRNA_101996 exerts important roles in various cancers. Our previous studies showed that circRNA_101996 promoted cervical cancer growth and metastasis by regulating miR-8075/TPX2. However, the potential regulatory role of circRNA_101996 in cervical cancer still needs further investigation. Our results in this study suggested that circRNA_101996 was over-expressed in cervical cancer patients. circRNA_101996 up-regulation remarkably assisted cell proliferation, cell cycle progression, and cell migration in cervical cancer, while circRNA_101996 knockdown exerted the inverse effects. The molecular investigations indicated that circRNA_101996 could increase the expression level of miR-1236-3p, tripartite motif-containing 37 (TRIM37), through binding to miR-1236-3p and reducing its expression. Moreover, in vivo results demonstrated that circRNA_101996 shRNA can function as a tumor suppressor through down-regulating TRIM37 in cervical cancer. In conclusion, our data indicated that circRNA_101996/miR-1236-3p/TRIM37 axis accelerated cervical cancer development, providing novel insights into cervical cancer diagnosis and treatment.
环状 RNA（circRNAs）似乎是多种生理过程中的重要调节剂。最近发现，circRNA_101996 在多种癌症中发挥重要作用。我们之前的研究表明，circRNA_101996 通过调节 miR-8075/TPX2 促进宫颈癌的生长和转移。然而，circRNA_101996 在宫颈癌中的潜在调节作用仍需要进一步研究。本研究结果表明，circRNA_101996 在宫颈癌患者中过表达。circRNA_101996 的上调显著促进了宫颈癌细胞的增殖、细胞周期进程和细胞迁移，而 circRNA_101996 的下调则产生了相反的效果。分子研究表明，circRNA_101996 通过与 miR-1236-3p 结合并降低其表达水平，可增加 miR-1236-3p 和三结构域蛋白 37（TRIM37）的表达水平。此外，体内结果表明，circRNA_101996 shRNA 可通过下调宫颈癌中的 TRIM37 发挥肿瘤抑制作用。总之，我们的数据表明，circRNA_101996/miR-1236-3p/TRIM37 轴加速了宫颈癌的发展，为宫颈癌的诊断和治疗提供了新的见解。
