Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran 14117-13116, Iran.
Department of Biomedical Imaging & Image-guided Therapy, Division of Nuclear Medicine, Medical University of Vienna, Vienna, Austria.
Nanomedicine (Lond). 2021 Mar;16(7):553-567. doi: 10.2217/nnm-2020-0408. Epub 2021 Mar 17.
Here, we established a reliable strategy for generation and characterization of targeted radiolabeled exosomes for the detection of HER2-positive cells quantitatively. Targeted exosomes (T-exos) were radiolabeled by two different radiotracers, [Tc]Tc-HMPAO or [In]In-oxine. The labeling efficiency and stability were assessed using exosome exclusive spin columns. HER2-positive and -negative cells were treated with [In]In-oxine-exosomes after 3 and 24 h. [In]In-oxine labeling did not change the binding ability and general features of the exosomes. With [In]In-oxine, 70% labeling efficiency and 78% radiochemical stability over 24 h were achieved. [In]In-oxine-T-exos showed greater uptake by HER2-positive cells compared with untargeted exosomes. [In]In-oxine-T-exos could potentially be used as an effective imaging tool for HER2 expression.
在这里，我们建立了一种可靠的策略，用于生成和表征靶向放射性标记的外泌体，以便定量检测 HER2 阳性细胞。通过两种不同的放射性示踪剂[Tc]Tc-HMPAO 或[In]In-oxine 对靶向外泌体（T-exos）进行放射性标记。使用外泌体专用的螺旋柱评估标记效率和稳定性。用[In]In-oxine-exosomes 处理 HER2 阳性和阴性细胞 3 和 24 小时后。[In]In-oxine 标记不会改变外泌体的结合能力和一般特征。用[In]In-oxine，实现了 70%的标记效率和 24 小时内 78%的放射化学稳定性。与未靶向的外泌体相比，[In]In-oxine-T-exos 被 HER2 阳性细胞摄取更多。[In]In-oxine-T-exos 可能成为一种用于 HER2 表达的有效成像工具。
