巴西帕拉州海滩上售卖的牡蛎（Crassostrea spp.）中弓形虫的分子调查。
Molecular investigation of Toxoplasma gondii in oysters (Crassostrea spp.) sold on beaches in the State of Pará, Brazil.
作者信息
Rosário Marcely Karen Santos do, Silva Jacqueline, Melo Hugo Filipe Rodrigues, Monteiro Thamillys Rayssa Marques, Costa Davi Ribeiro da, Scofield Alessandra, Moraes Carla Cristina Guimarães de
机构信息
Laboratório de Zoonoses e Saúde Pública, Programa de Pós-graduação em Saúde Animal na Amazônia, Instituto de Medicina Veterinária, Universidade Federal do Pará, Castanhal - UFPA, Castanhal, PA, Brasil.
Laboratório de Patologia Animal, Programa de Pós-graduação em Saúde Animal na Amazônia, Instituto de Medicina Veterinária, Universidade Federal do Pará - UFPA, Castanhal, PA, Brasil.
出版信息
Rev Bras Parasitol Vet. 2021 Mar 12;30(1):e023320. doi: 10.1590/S1984-29612021004. eCollection 2021.
The aim of this study was to detect Toxoplasma gondii DNA in oysters (Crassostrea spp.) sold on seven beaches in the State of Pará, Brazil. According to the National Program for Hygiene and Sanitary Control of Bivalve Mollusks, 100 g of the edible part of mollusks is required to analyze contaminating microorganisms. In this study, 12 oysters were assumed to be equivalent to 100 g of edible parts when preparing each pooled sample. In total, 360 oysters were purchased from 30 vendors. From groups of 12 oysters purchased per vendor, 60 pooled samples were obtained, comprising 30 gill tissues and 30 gastrointestinal tracts. For molecular analysis, nested-PCR was conducted to amplify a 155-base-pair product of the B1 gene from T. gondii. All analyzed samples were negative for T. gondii. Our findings indicate that the oyster samples sold on the beaches in the State of Pará were not contaminated by T. gondii.
本研究的目的是检测在巴西帕拉州七个海滩上售卖的牡蛎（Crassostrea spp.）中的刚地弓形虫DNA。根据双壳贝类国家卫生与卫生控制计划，分析污染微生物需要100克贝类的可食用部分。在本研究中，制备每个混合样本时，假定12个牡蛎相当于100克可食用部分。总共从30个摊贩处购买了360个牡蛎。从每个摊贩购买的12个牡蛎组中，获得了60个混合样本，包括30个鳃组织样本和30个胃肠道样本。为了进行分子分析，采用巢式PCR扩增来自刚地弓形虫的B1基因的155个碱基对的产物。所有分析样本的刚地弓形虫检测均为阴性。我们的研究结果表明，在帕拉州海滩上售卖的牡蛎样本未被刚地弓形虫污染。
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