Unità di Microbiologia, Bambino Gesù, Ospedale Pediatrico e Istituto di Ricerca, Piazza Sant'Onofrio 4, 00165 Roma, Italy.
Exp Parasitol. 2011 Feb;127(2):409-17. doi: 10.1016/j.exppara.2010.09.007. Epub 2010 Oct 25.
To evaluate the presence of Toxoplasma gondii in edible farmed shellfish, 1734 shellfish specimens i.e., 109 Crassostrea gigas (6 pools), 660 Mytilus galloprovincialis (22 pools), 804 Tapes decussatus (28 pools) and 161 Tapes philippinarum (6 pools), were collected from the Varano Lagoon (Apulia, Italy). Shellfish from 62 pools were subjected to two molecular techniques: a nested-PCR assay, and a fluorescent amplicon generation (FLAG) real-time PCR assay, both based on the multi-copy B1 target, were performed. One pooled sample of gills from C. gigas and one pooled sample of haemolymphs from T. decussatus were assessed as positive for T. gondii DNA by both techniques. The results demonstrated the presence of T. gondii in edible farmed C. gigas and T. decussatus and indicate that there may be a considerable health threat involved in eating contaminated raw shellfish.
为评估食源性养殖贝类中刚地弓形虫的存在情况，从意大利普利亚的拉古纳·瓦拉诺（Varano Lagoon）采集了 1734 个贝类标本，包括 109 个巨蛎（Crassostrea gigas，6 个贝柱）、660 个贻贝（Mytilus galloprovincialis，22 个贝柱）、804 个帘蛤（Tapes decussatus，28 个贝柱）和 161 个菲律宾蛤仔（Tapes philippinarum，6 个贝柱）。62 个贝柱样本采用两种分子技术进行检测：巢式 PCR 检测和荧光扩增子生成（FLAG）实时 PCR 检测，均基于多拷贝 B1 靶标进行。两种技术均检测到来自 C. gigas 的鳃部混合样本和来自 T. decussatus 的血淋巴混合样本中存在刚地弓形虫 DNA。结果表明，食源性养殖的 C. gigas 和 T. decussatus 中存在刚地弓形虫，这表明食用受污染的生贝类可能存在相当大的健康威胁。
