School of Agriculture Food and Wine, University of Adelaide, Glen Osmond, Australia.
Pest Manag Sci. 2021 Jul;77(7):3080-3087. doi: 10.1002/ps.6367. Epub 2021 Apr 1.
Gene amplification has been shown to provide resistance to glyphosate in several weed species, including Hordeum glaucum populations in South Australia. The stability of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene copies in resistant populations in the presence or absence of glyphosate selection has not been determined.
Applying glyphosate to a cloned plant resulted in an increase in resistance and EPSPS copy number in the progeny of that plant compared to the untreated clone. The LD (herbicide concentration required for 50% mortality) increased by 75% to 79% in the progeny of the treated clones compared to the untreated in both populations (YP-17 and YP-16). EPSPS copy number estimates were higher in treated individuals compared to untreated individuals with an average of seven copies compared to six in YP-16 and 11 compared to six in YP-17. There was a positive correlation (R = 0.78) between EPSPS copy number and LD of all populations.
EPSPS gene copy number and resistance to glyphosate increased in H. glaucum populations under glyphosate selection, suggesting the number of EPSPS gene copies present is dependent on glyphosate selection. © 2021 Society of Chemical Industry.
基因扩增已被证明可使几种杂草物种（包括南澳大利亚的大麦种群）对草甘膦产生抗性。在存在或不存在草甘膦选择的情况下，抗性种群中 5-烯醇丙酮酰莽草酸-3-磷酸合酶（EPSPS）基因拷贝的稳定性尚未确定。
将草甘膦应用于克隆植物会导致该植物后代的抗性和 EPSPS 拷贝数增加，与未处理的克隆相比。与未处理的克隆相比，处理过的克隆的 LD（导致 50%死亡率的除草剂浓度）在两个种群（YP-17 和 YP-16）中分别增加了 75%至 79%。与未处理的个体相比，处理过的个体的 EPSPS 拷贝数估计值更高，YP-16 中平均有 7 个拷贝，而 YP-16 中平均有 6 个拷贝，YP-17 中平均有 11 个拷贝，而 YP-17 中平均有 6 个拷贝。所有种群的 EPSPS 拷贝数和 LD 之间存在正相关（R = 0.78）。
在草甘膦选择下，大麦种群的 EPSPS 基因拷贝数和对草甘膦的抗性增加，表明 EPSPS 基因拷贝数的存在取决于草甘膦的选择。 © 2021 化学工业协会。
