School of Agriculture Food and Wine, University of Adelaide, PMB 1, Glen Osmond, SA, Australia.
Pest Manag Sci. 2020 Apr;76(4):1214-1221. doi: 10.1002/ps.5671. Epub 2019 Dec 2.
Glyphosate failed to control two populations of Hordeum glaucum (northern barley grass) along a fence line and around stockyards near Arthurton on the Yorke Peninsula, South Australia after more than a decade of regular use of glyphosate. These were investigated to confirm resistance and to determine resistance mechanisms.
Dose-response experiments confirmed resistance of these populations to glyphosate with resistance levels of 2.8-6.6-fold compared with two susceptible populations. Shikimate assays confirmed resistance to glyphosate with less shikimate accumulation in the resistant populations compared with the susceptible populations. Quantitative PCR of genomic DNA showed increased gene copy number in the resistant populations with 9-11-fold more copies of EPSPS compared with the susceptible populations, suggesting resistance is likely conferred by gene amplification.
This study identified the first examples of glyphosate resistance in the grass species H. glaucum with resistance associated with EPSPS gene amplification. © 2019 Society of Chemical Industry.
在南澳大利亚约克半岛阿瑟顿附近的围栏沿线和牲畜围场，草甘膦十多年来一直定期使用，但仍未能控制两种大麦草（北方大麦草）种群。对这些种群进行了调查，以确认其对草甘膦的抗性，并确定其抗性机制。
剂量反应实验证实，这些种群对草甘膦具有抗性，与两个敏感种群相比，抗性水平为 2.8-6.6 倍。莽草酸测定证实，与敏感种群相比，抗性种群中草甘膦的莽草酸积累减少。对基因组 DNA 的定量 PCR 显示，抗性种群中 EPSPS 基因的拷贝数增加，与敏感种群相比，增加了 9-11 倍，表明抗性可能是由基因扩增引起的。
本研究首次在禾本科植物大麦草中发现了草甘膦抗性的例子，抗性与 EPSPS 基因扩增有关。 © 2019 化学工业协会。
