光热疗法诱导黑素瘤细胞死亡机制的转录组学分析。
Transcriptomic analysis of mechanism of melanoma cell death induced by photothermal therapy.
机构信息
Institute of Photomedicine, Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai, China.
Stephenson School of Biomedical Engineering, Gallogly College of Engineering, University of Oklahoma, Norman, Oklahoma, USA.
出版信息
J Biophotonics. 2021 Aug;14(8):e202100034. doi: 10.1002/jbio.202100034. Epub 2021 May 26.
Melanoma is a malignancy with poor prognosis. Its incidence rate has been on the rise and it poses high health and economic challenges to different populations. Photothermal therapy (PTT) served as an effective local therapy in treating various tumors, particularly cutaneous carcinoma like melanoma. To fully understand the mechanisms of tumor cell death induced by PTT, we investigated gene expression and immune cells compositions of B16-F10 tumors after PTT treatment. A total of 256 differentially expressed genes (DEGs) were identified, with 215 being downregulated and 41 upregulated by PTT. Functional annotation showed that most DEGs involved in immune response and inflammatory response. Immune cells compositions inference revealed changes in many immune cells including regulatory T cells, M2 macrophage and B cells after PTT treatment. Our results help delineate the mechanism of cell death at the transcriptional level triggered by non-invasive PTT treatment of melanoma without exogenous light absorbing agents.
黑色素瘤是一种预后不良的恶性肿瘤。其发病率一直在上升，给不同人群的健康和经济带来了巨大挑战。光热疗法（PTT）是治疗各种肿瘤的一种有效局部治疗方法，特别是皮肤癌如黑色素瘤。为了充分了解 PTT 诱导的肿瘤细胞死亡的机制，我们研究了 PTT 治疗后 B16-F10 肿瘤的基因表达和免疫细胞组成。鉴定出了 256 个差异表达基因（DEGs），其中 215 个基因经 PTT 下调，41 个基因上调。功能注释表明，大多数 DEGs 参与免疫反应和炎症反应。免疫细胞组成推断显示，PTT 治疗后，许多免疫细胞发生了变化，包括调节性 T 细胞、M2 巨噬细胞和 B 细胞。我们的研究结果有助于在没有外源光吸收剂的情况下，从转录水平上阐明非侵入性 PTT 治疗黑色素瘤所引发的细胞死亡机制。
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