Jetté L P, Lambert N G
Laboratoire de Santé Publique du Québec, Sainte-Anne-de-Bellevue, Canada.
Infect Control Hosp Epidemiol. 1988 May;9(5):194-9. doi: 10.1086/645832.
Two models of hot water washer disinfectors (Decomat 128 and Hospital A, Euroclean Canada Inc; Ontario, Canada) were evaluated by two methods for their efficacy in disinfecting anesthesia equipment. In the first method, three different microbial suspensions were each sealed into 30 capillary tubes. In the second method, corrugated anesthesia tubes were rinsed with suspensions of each of two bacterial strains. The tubes then underwent a standard cycle in the hot water washer disinfectors and were subsequently tested for growth of microorganisms. All experiments were repeated three times, and the temperature was registered in all cases. In the capillary test, growth was rarely detected (13/540 tubes) and the inactivation factor for both apparatus was greater than 5 log10. In the rinse test, no growth was detected. The mean temperature for 15 disinfection cycles was 84.2 +/- 0.8 degrees C for Decomat 128 and 88.9 +/- 0.5 degrees C for Hospital A. However, for Decomat 128 we observed a variation of 3 degrees C from one disinfection cycle to another and a progressive reduction of 2.2 degrees C over a series of five consecutive complete cycles. Both methods gave reproducible results. Under our experimental conditions, both hot water washer disinfectors proved to be efficacious for the disinfection of reusable anesthesia equipment.
通过两种方法对两种型号的热水清洗消毒器(Decomat 128和加拿大安大略省Euroclean Canada Inc公司的Hospital A)对麻醉设备的消毒效果进行了评估。在第一种方法中,将三种不同的微生物悬液分别密封在30根毛细管中。在第二种方法中,用两种细菌菌株的悬液冲洗波纹状麻醉管。然后将这些管子在热水清洗消毒器中进行标准循环,随后检测微生物的生长情况。所有实验均重复三次,并记录所有情况下的温度。在毛细管试验中,很少检测到生长(540根管子中有13根),两种设备的灭活因子均大于5个对数10。在冲洗试验中,未检测到生长。Decomat 128的15个消毒循环的平均温度为84.2±0.8℃,Hospital A的为88.9±0.5℃。然而,对于Decomat 128,我们观察到从一个消毒循环到另一个消毒循环的温度变化为3℃,并且在一系列连续的五个完整循环中温度逐渐降低2.2℃。两种方法都给出了可重复的结果。在我们的实验条件下,两种热水清洗消毒器都被证明对可重复使用的麻醉设备消毒有效。