Dusseau J Y, Luu Duc D, Foissaud V, Marchetti B, Ducel G, Clavier B, Calop J
Laboratoire de Biologie médicale, Hôpital d'instruction des armées Desgenettes, Lyon, France.
Pathol Biol (Paris). 2001 Feb;49(1):23-32. doi: 10.1016/s0369-8114(00)00001-8.
The authors studied seven automatic washer disinfectors for flexible endoscopes with two methods. The first method, a microdilution method, studied the bactericidal activity of the seven disinfectants against 21 strains: four reference strains, 14 hospital strains reported in the literature as nosocomial strains responsible for infections transmitted by flexible endoscopes and three vancomycin-resistant Enterococci. The ability of the seven automatic washer disinfectors to decontaminate flexible endoscopes following inoculation with four reference strains was studied with the second method. There were three kinds of results: the results of three automatic washer disinfectors in conformity with both methods; the results of three automatic washer disinfectors conformity with 1 method only; the results of one automatic washer disinfector without conformity with any methods. Both methods should be used for evaluation of automatic washer disinfector. Then, these results emphasize the necessity to modify the use of disinfectants and/or the systems of some automatic washer disinfectors.
作者用两种方法研究了七台用于软式内镜的自动清洗消毒机。第一种方法是微量稀释法，研究了这七种消毒剂对21株菌株的杀菌活性：四株参考菌株、14株文献报道的医院菌株（这些医院菌株被认为是由软式内镜传播感染的医院内感染菌株）以及三株耐万古霉素肠球菌。第二种方法研究了在接种四株参考菌株后，这七台自动清洗消毒机对软式内镜进行去污处理的能力。结果有三种：三台自动清洗消毒机的结果在两种方法中均符合要求；三台自动清洗消毒机的结果仅符合一种方法；一台自动清洗消毒机的结果不符合任何方法。两种方法均应用于评估自动清洗消毒机。然后，这些结果强调了修改消毒剂使用方法和/或一些自动清洗消毒机系统的必要性。
