Yarosh D B, Yee V
Applied Genetics Inc., Freeport, NY 11520.
J Photochem Photobiol B. 1990 Nov;7(2-4):173-9. doi: 10.1016/1011-1344(90)85154-o.
The SKH-1 hairless mouse strain has been used extensively as a model for human photocarcinogenesis, photoimmunology and photoaging, but little is known about DNA repair in living mouse skin. Mice were irradiated with UV-B light at doses which produce mild to severe sunburn, and the frequency of pyrimidine dimers in epidermal DNA was measured immediately and 6 h after irradiation using T4 endonuclease V treatment and alkaline agarose gel electrophoresis. The results demonstrate significant removal of pyrimidine dimers in mouse skin in vivo, with a dimer half-life of 7.4 h. These findings are similar to the repair of dimers in human skin in vivo. The SKH-1 hairless mouse is thus a useful model for pyrimidine dimer repair in human skin.
SKH-1无毛小鼠品系已被广泛用作人类光致癌作用、光免疫学和光老化的模型，但对于活体小鼠皮肤中的DNA修复却知之甚少。用能引起轻度至重度晒伤的剂量的UV-B光照射小鼠，使用T4内切核酸酶V处理和碱性琼脂糖凝胶电泳，在照射后立即和6小时测量表皮DNA中嘧啶二聚体的频率。结果表明，小鼠皮肤中的嘧啶二聚体在体内有显著清除，二聚体半衰期为7.4小时。这些发现与人类皮肤中二聚体的体内修复情况相似。因此，SKH-1无毛小鼠是人类皮肤中嘧啶二聚体修复的有用模型。
