Department of Chemistry and State Key Laboratory of Synthetic Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China, Laboratory for Synthetic Chemistry and Chemical Biology, Health@InnoHK, Innovation and Technology Commission, Hong Kong, SAR, China.
Chem Commun (Camb). 2021 Apr 21;57(31):3769-3772. doi: 10.1039/d1cc00961c. Epub 2021 Mar 17.
Membrane proteins are important drug targets; however, ligand discovery for membrane proteins is highly challenging due to their hydrophobic nature. We show that membrane proteins may be specifically labelled with a DNA tag by DNA-programmed affinity labelling (DPAL), thereby enabling the screening of chemical compounds against membrane proteins directly on live cells.
膜蛋白是重要的药物靶点；然而，由于其疏水性，膜蛋白的配体发现极具挑战性。我们表明，膜蛋白可以通过 DNA 编程亲和标记 (DPAL) 进行特异性标记，从而可以直接在活细胞上针对膜蛋白筛选化学化合物。
