Pan Y Y, Yang X, Cao L J, Liu Q, Xiong Y W
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2021 Mar 6;55(3):365-370. doi: 10.3760/cma.j.cn112150-20200330-00469.
To evaluate the antimicrobial resistance of non-O157 Shiga toxin-producing (STEC) isolated from human cases. From 2011 to 2019, 33 non-O157 STEC strains recovered from diarrheal patients from 7 provinces/cities were collected, including Qinghai (1 isolate), Heilongjiang (1 isolate), Guangxi (2 isolates), Shandong (2 isolates), Guangdong (4 isolates), Henan (11 isolates), and Shanghai (12 isolates). Minimum inhibitory concentration (MIC) of 19 antimicrobials were tested by broth microdilution method; O∶H serotypes, Multi-locus sequence typing (MLST) and antimicrobial resistance genes were determined by whole genome sequencing. A total of 33 non-O157 STEC strains were typed into 19 O∶H serotypes and 17 sequence types (STs), respectively. Ten strains were resistant to one or more antibiotics,of which five were multiple drug-resistant (MDR). The resistance rate of tetracycline was 30.3% (10 isolates), and azithromycin resistant strains were detected (12.12%, 4 isolates), but all strains were susceptible to carbapenems. All strains carried the gene, and the Extended-Spectrum β-Lactamase (ESBL) genotype were detected (3.0%, 1 isolates). The gene was firstly detected in non-O157 STEC strains. MDR, azithromycin resistance, and multiple drug resistance genes were detected in human-derived non-O157 STECs in many regions in China, but they were all susceptible to carbapenems. Our results might guide the clinical treatment of STEC infections.
评估从人类病例中分离出的非O157产志贺毒素大肠杆菌（STEC）的抗菌耐药性。2011年至2019年，收集了从7个省/市腹泻患者中分离出的33株非O157 STEC菌株，包括青海（1株）、黑龙江（1株）、广西（2株）、山东（2株）、广东（4株）、河南（11株）和上海（12株）。采用肉汤微量稀释法检测19种抗菌药物的最低抑菌浓度（MIC）；通过全基因组测序确定O∶H血清型、多位点序列分型（MLST）和抗菌耐药基因。共33株非O157 STEC菌株分别被分型为19种O∶H血清型和17种序列类型（STs）。10株菌株对一种或多种抗生素耐药，其中5株为多重耐药（MDR）。四环素耐药率为30.3%（10株），检测到阿奇霉素耐药菌株（12.12%，4株），但所有菌株对碳青霉烯类药物敏感。所有菌株均携带该基因，检测到超广谱β-内酰胺酶（ESBL）基因型（3.0%），1株。该基因首次在非O157 STEC菌株中检测到。在中国多个地区的人源非O157 STEC中检测到MDR、阿奇霉素耐药和多种耐药基因，但它们对碳青霉烯类药物均敏感。我们的结果可能为STEC感染的临床治疗提供指导。
