二烯丙基三硫化物通过NF-κB途径抑制烟草烟雾介导的膀胱上皮-间质转化和癌症干细胞标志物表达。

Diallyl trisulfide inhibited tobacco smoke-mediated bladder EMT and cancer stem cell marker expression via the NF-κB pathway .

作者信息

Geng Hao, Guo Wenhao, Feng Lei, Xie Dongdong, Bi Liangkuan, Wang Yi, Zhang Tao, Liang Zhaofeng, Yu Dexin

机构信息

Department of Urology, Second Affiliated Hospital of Anhui Medical University, Hefei, China.

Key Laboratory of Medical Science and Laboratory Medicine of Jiangsu Province, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.

出版信息

J Int Med Res. 2021 Mar;49(3):300060521992900. doi: 10.1177/0300060521992900.

OBJECTIVE

This study examined the effect of the NF-κB pathway on tobacco smoke-elicited bladder epithelial-mesenchymal transition (EMT) and cancer stem cell (CSC) marker expression . The effect of diallyl trisulfide (DATS) treatment was also examined.

METHODS

BALB/c mice were exposed to tobacco smoke and treated with an NF-κB inhibitor and DATS. Western blotting, quantitative real-time PCR, and immunohistochemical staining were used to detect the changes of relevant indices.

RESULTS

Phosphorylated inhibitor of kappa-B kinase alpha/beta expression and p65 and p50 nuclear transcription were increased by tobacco smoke exposure, whereas inhibitor of kappa-B expression was decreased. In addition, tobacco smoke reduced the expression of epithelial markers but increased that of mesenchymal and CSC markers. Our study further demonstrated that tobacco smoke-mediated EMT and CSC marker expression were attenuated by inhibition of the NF-κB pathway. Moreover, DATS reversed tobacco smoke-induced NF-κB pathway activation, EMT, and the acquisition of CSC properties in bladder tissues.

CONCLUSIONS

These data suggested that the NF-κB pathway regulated tobacco smoke-induced bladder EMT, CSC marker expression, and the protective effects of DATS.

目的

本研究探讨核因子κB（NF-κB）信号通路对烟草烟雾诱导的膀胱上皮-间质转化（EMT）及癌症干细胞（CSC）标志物表达的影响。同时也研究了二烯丙基三硫醚（DATS）处理的效果。

方法

将BALB/c小鼠暴露于烟草烟雾中，并用NF-κB抑制剂和DATS进行处理。采用蛋白质免疫印迹法、定量实时聚合酶链反应和免疫组织化学染色检测相关指标的变化。

结果

烟草烟雾暴露可使磷酸化的κB激酶α/β抑制剂表达以及p65和p50核转录增加，而κB抑制剂表达降低。此外，烟草烟雾降低了上皮标志物的表达，但增加了间质标志物和CSC标志物的表达。我们的研究进一步表明，抑制NF-κB信号通路可减弱烟草烟雾介导的EMT和CSC标志物表达。此外，DATS可逆转烟草烟雾诱导的膀胱组织中NF-κB信号通路激活、EMT及CSC特性的获得。