Department of Biochemistry, University of Port Harcourt, Choba, Nigeria.
Drug Chem Toxicol. 2022 Jul;45(4):1881-1890. doi: 10.1080/01480545.2021.1892949. Epub 2021 Mar 18.
Here, we studied the protective effect of gallic acid (GAL) as a potent anti-oxidant and anti-inflammatory agent against damage caused by busulfan (BUS) in the testes of adult rats. The adult Wistar rats were assigned as control, BUS: was intraperitoneally () treated with busulfan (15mg/kg, day 7 and 14), GAL + BUS: was co-treated with busulfan ( 15mg/kg, day 7 and 14) and orally treated ( with gallic acid (60days, 20mg/kg) and GAL: was treated with gallic acid (, 60days, 20mg/kg). The results showed that GAL co-treatment increased the numbers of spermatogonia (Type A and B), spermatocytes (primary and secondary) and round spermatids, along with the tubular diameter, epithelial height and gonado-somatic index. In addition, BUS-induced increase in 3β-hydroxysteroid dehydrogenase and γ-glutamyl transpeptidase activities were inhibited on GAL co-treatment. Similarly, BUS-induced decrease in gluthathione concentration, catalase and superoxide dismutase activities along with increase in myeloperoxidase activity and malondialdehyde concentration were significantly normalized to control values on GAL co-treatment. Busulfan-induced elimination of tubular germ cells was completely prevented by GAL. Overall, GAL may inhibit BUS-mediated spermatogenesis arrest via decreasing inflammatory-mediated oxidative stress in a rat experimental model.
在这里，我们研究了没食子酸（GAL）作为一种有效的抗氧化剂和抗炎剂对成年大鼠睾丸中白消安（BUS）引起的损伤的保护作用。成年 Wistar 大鼠被分为对照组、BUS 组：腹腔内（）用白消安（15mg/kg，第 7 天和第 14 天）处理、GAL+BUS 组：用白消安（15mg/kg，第 7 天和第 14 天）共同处理和口服（用没食子酸（60 天，20mg/kg）处理、GAL 组：用没食子酸（60 天，20mg/kg）处理。结果表明，GAL 共同处理增加了精原细胞（A 型和 B 型）、精母细胞（初级和次级）和圆形精子细胞的数量，以及管状直径、上皮高度和性腺体指数。此外，BUS 诱导的 3β-羟甾脱氢酶和γ-谷氨酰转肽酶活性的增加在 GAL 共同处理时受到抑制。同样，BUS 诱导的谷胱甘肽浓度、过氧化氢酶和超氧化物歧化酶活性的降低以及髓过氧化物酶活性和丙二醛浓度的增加在 GAL 共同处理时与对照组相比显著正常化。GAL 完全阻止了 BUS 诱导的管状生殖细胞消除。总的来说，GAL 可能通过降低大鼠实验模型中炎症介导的氧化应激来抑制 BUS 介导的精子发生停滞。
