Abarikwu Sunny O, Durojaiye Mojisola, Alabi Adenike, Asonye Bede, Akiri Oghenetega
a Department of Biochemistry , University of Port Harcourt , East-West Road , Choba , Nigeria ;
b Department of Chemical Sciences , Redeemer's University , Redemption City , Ogun State , Nigeria.
Ren Fail. 2016;38(2):321-9. doi: 10.3109/0886022X.2015.1127743. Epub 2015 Dec 27.
Curcumin (Cur) and gallic acid (Gal) are major food additives. Cur has well-known antioxidant properties, whereas Gal has both antioxidant and pro-oxidant effects. The present study investigated the effects of oral administration of Gal with or without Cur on antioxidant enzymes activities, glutathione (GSH) and the enzymes in its metabolism in rat liver in vivo and markers of tissue damage in the serum. Results showed that the increase in serum creatinine level, alkaline phosphatase and lactate dehydrogenase activities by Gal treatment were inhibited by combined administration of Gal and Cur. The decrease in GSH-peroxidase, GSH-S-transferase, superoxide dismutase and GSH-reductase activities by Gal treatment were inhibited when both Gal and Cur were administered together. The malondialdehyde concentration and catalase activity were significantly increased following administration of Gal but not when the administration of Gal was combined with Cur. Finally, the increase in GSH level was seen following administration of Cur alone or in combination with Gal but not with Gal alone. These results suggest that Gal might induce oxidative stress in the rat liver and affect renal function that can be inhibited by the combined administration of Gal and Cur.
姜黄素（Cur）和没食子酸（Gal）是主要的食品添加剂。姜黄素具有众所周知的抗氧化特性，而没食子酸兼具抗氧化和促氧化作用。本研究调查了口服给予没食子酸单独或与姜黄素联合使用对大鼠肝脏抗氧化酶活性、谷胱甘肽（GSH）及其代谢中的酶以及血清中组织损伤标志物的影响。结果表明，联合给予没食子酸和姜黄素可抑制没食子酸处理导致的血清肌酐水平、碱性磷酸酶和乳酸脱氢酶活性升高。同时给予没食子酸和姜黄素时，可抑制没食子酸处理导致的谷胱甘肽过氧化物酶、谷胱甘肽 - S - 转移酶、超氧化物歧化酶和谷胱甘肽还原酶活性降低。单独给予没食子酸后丙二醛浓度和过氧化氢酶活性显著升高，但没食子酸与姜黄素联合给药时则未出现此现象。最后，单独给予姜黄素或姜黄素与没食子酸联合给药后谷胱甘肽水平升高，而单独给予没食子酸时未出现此现象。这些结果表明，没食子酸可能在大鼠肝脏中诱导氧化应激并影响肾功能，而没食子酸与姜黄素联合给药可抑制这种影响。
