Laboratório De Mutagênese, Universidade De Franca, Franca, São Paulo, Brazil.
Facudade De Ciências Farmacêuticas De Ribeirão Preto, Universidade De São Paulo, São Paulo, Brazil.
J Toxicol Environ Health A. 2021 Jul 18;84(14):569-581. doi: 10.1080/15287394.2021.1898505. Epub 2021 Mar 17.
Desf. is a plant found in South America, especially in Brazil. Oleoresin and the leaves of this plant is used as a popular medicinal agent. However, few studies on the chemical composition of aerial parts and related biological activities are known. This study aimed to examine the cytotoxic, genotoxic, and antigenotoxic potential of aerial parts hydroalcoholic extract (CLE) and two of its major compounds afzelin and quercitrin. The cytotoxic and antigenotoxic potential of CLE was determined as follows: 1) against genotoxicity induced by doxorubicin (DXR) or methyl methanesulfonate (MMS) in V79 cells; 2) by direct and indirect-acting mutagens in strains; and 3) by MMS in male Swiss mice. The protective effects of afzelin and quercitrin against DXR or MMS were also evaluated in V79 and HepG2 cells. CLE was cytotoxic as evidenced by clonogenic efficiency assay. Further, CLE did not induce a significant change in frequencies of chromosomal aberrations and micronuclei; as well as number of revertants in the Ames test demonstrating absence of genotoxicity. In contrast, CLE was found to be antigenotoxic in mammalian cells. The results also showed that CLE exerted inhibitory effect against indirect-acting mutagens in the Ames test. Afzelin and quercitrin did not reduce genotoxicity induced by DXR or MMS in V79 cells. However, treatments using afzelin and quercitrin decreased MMS-induced genotoxicity in HepG2 cells. The antigenotoxic effect of CLE observed in this study may be partially attributed to the antioxidant activity of the combination of major components afzelin and quercitrin.
德斯芬是一种生长在南美洲的植物，特别是在巴西。这种植物的树脂和叶子被用作一种流行的药用制剂。然而，人们对其地上部分的化学成分和相关生物活性的研究知之甚少。本研究旨在考察地上部分水醇提取物（CLE）及其两种主要化合物飞燕草素和槲皮素的细胞毒性、遗传毒性和抗原毒性潜力。通过以下方法确定 CLE 的细胞毒性和抗原毒性潜力：1）对 V79 细胞中阿霉素（DXR）或甲磺酸甲酯（MMS）诱导的遗传毒性的影响；2）对菌株中直接和间接致突变物的影响；3）MMS 对雄性瑞士小鼠的影响。还评估了飞燕草素和槲皮素对 V79 和 HepG2 细胞中 DXR 或 MMS 的保护作用。CL 表现出细胞毒性，这一点可以从集落形成效率测定中得到证明。此外，CLE 并未导致染色体畸变和微核频率发生显著变化；Ames 试验中的回复突变数也表明其没有遗传毒性。相反，CLE 在哺乳动物细胞中被发现具有抗原毒性。结果还表明，CLE 对 Ames 试验中的间接致突变物具有抑制作用。飞燕草素和槲皮素并不能降低 V79 细胞中 DXR 或 MMS 诱导的遗传毒性。然而，使用飞燕草素和槲皮素的治疗降低了 HepG2 细胞中 MMS 诱导的遗传毒性。本研究观察到的 CLE 的抗原毒性作用可能部分归因于主要成分飞燕草素和槲皮素的抗氧化活性。
