Czuppon A B
Dept Obstetrics and Gynaecology, University of Kiel, FRG.
J Clin Chem Clin Biochem. 1988 Jan;26(1):35-8.
A new commercially available Sperm Antibody Slide-Test kit for the detection of antibodies to sperm (Biotec Diagnostica International GmbH, Hannover, FRG) was evaluated with respect to its diagnostic value in immunological infertility. The new test correlates better with clinical findings than the sperm-agglutination and sperm-immobilization assays. Compared with the other two tests, it shows a higher percentage of positive reactions with infertile patients. None of the 100 fertile subjects was positive in the Sperm Antibody Slide-Test, as opposed to approx. 13% false positives by the conventional methods. In addition, the new test is easier to perform and requires no vital spermatozoa. Since the kit is stable for over 12 months at 4-8 degrees C, requires no spermatozoal preparations, and has a relatively quick reaction time of 2-5 minutes, it is well suited for the routine diagnosis of patients with immunological infertility problems.
对一种新上市的用于检测精子抗体的精子抗体玻片检测试剂盒（德国汉诺威的Biotec Diagnostica International GmbH公司生产）在免疫性不孕诊断价值方面进行了评估。与精子凝集试验和精子制动试验相比，这种新检测方法与临床结果的相关性更好。与其他两种检测方法相比，它在不孕患者中显示出更高的阳性反应百分比。100名有生育能力的受试者在精子抗体玻片检测中均为阴性，而传统方法约有13%的假阳性。此外，这种新检测方法操作更简便，不需要活精子。由于该试剂盒在4至8摄氏度下可稳定保存12个月以上，无需精子制备，且反应时间相对较快，为2至5分钟，因此非常适合用于免疫性不孕问题患者的常规诊断。
