Departments of Pharmaceutical Sciences.
Medicine.
Dermatol Surg. 2021 Jul 1;47(7):944-946. doi: 10.1097/DSS.0000000000002990.
Croton oil (CO) is used by dermatologists and plastic surgeons in deep chemical peels. It is mixed with phenol, water, and a soap in Baker-Gordon's or Hetter's formulas. There is controversy as to whether CO or phenol is the active agent in the dermal effect of deep chemical peels.
To better clarify the role of CO in deep peels, by identification of active compounds in commercially available CO in the United States and biological effects in vivo.
Liquid chromatography-tandem mass spectrometry on CO and a domestic pig model experiment using 3 different formulas: G1: 5% Septisol (SEP), G2: 1.6% croton oil in 35% phenol with 5% SEP, and G3: 35% phenol with 5% SEP.
Liquid chromatography-tandem mass spectrometry indicated the presence of phorbol esters. G1 was null overall. Extent of the coagulative necrosis: G2 > G3. Vascular ectasia: G2 > G3. Inflammation pattern: intense neutrophilic inflammatory band in G2 versus mild, sparse, perivascular mononuclear cell infiltrate in G3. Neocollagenesis: pronounced in G2, negligible in G3.
Coagulative necrosis of the epidermis, superficial fibroblasts, and vasculature can be attributed to the action of phenol. Phorbol esters on CO could be responsible for the dense deep acute inflammation and the distinctive neocollagenesis.
皮肤科医生和整形外科医生在深层化学换肤中使用巴豆油（CO）。它与苯酚、水和肥皂混合在贝克 - 戈登或赫特的配方中。CO 还是苯酚是深层化学换肤真皮效应中的活性物质存在争议。
通过鉴定美国市售 CO 中的活性化合物和体内生物学效应，更好地阐明 CO 在深层换肤中的作用。
对 CO 进行液相色谱-串联质谱分析，并在国内猪模型上使用 3 种不同的配方进行实验：G1：5%Septisol（SEP），G2：35%苯酚中的 1.6%巴豆油加 5%SEP，G3：35%苯酚加 5%SEP。
液相色谱-串联质谱分析表明存在佛波酯。G1 总体上为阴性。凝固性坏死程度：G2>G3。血管扩张：G2>G3。炎症模式：G2 中强烈的中性粒细胞炎症带，G3 中轻度、稀疏、血管周围单核细胞浸润。新胶原形成：G2 明显，G3 可忽略不计。
表皮、浅层成纤维细胞和血管的凝固性坏死可归因于苯酚的作用。CO 中的佛波酯可能是导致密集的深层急性炎症和独特的新胶原形成的原因。
