Slovenian Forestry Institute, Večna pot 2, 1000, Ljubljana, Slovenia.
Department of Plant, Soil, and Microbial Sciences, Michigan State University, 426 Auditorium Road, East Lansing, MI, 48824, USA.
Sci Rep. 2021 Mar 17;11(1):6167. doi: 10.1038/s41598-021-85497-8.
Species of the genus Tuber have gained a lot of attention in recent decades due to their aromatic hypogenous fruitbodies, which can bring high prices on the market. The tendency in truffle production is to infect oak, hazel, beech, etc. in greenhouse conditions. We aimed to show whether silver fir (Abies alba Mill.) can be an appropriate host partner for commercial mycorrhization with truffles, and how earthworms in the inoculation substrate would affect the mycorrhization dynamics. Silver fir seedlings inoculated with Tuber. aestivum were analyzed for root system parameters and mycorrhization, how earthworms affect the bare root system, and if mycorrhization parameters change when earthworms are added to the inoculation substrate. Seedlings were analyzed 6 and 12 months after spore inoculation. Mycorrhization with or without earthworms revealed contrasting effects on fine root biomass and morphology of silver fir seedlings. Only a few of the assessed fine root parameters showed statistically significant response, namely higher fine root biomass and fine root tip density in inoculated seedlings without earthworms 6 months after inoculation, lower fine root tip density when earthworms were added, the specific root tip density increased in inoculated seedlings without earthworms 12 months after inoculation, and general negative effect of earthworm on branching density. Silver fir was confirmed as a suitable host partner for commercial mycorrhization with truffles, with 6% and 35% mycorrhization 6 months after inoculation and between 36% and 55% mycorrhization 12 months after inoculation. The effect of earthworms on mycorrhization of silver fir with Tuber aestivum was positive only after 6 months of mycorrhization, while this effect disappeared and turned insignificantly negative after 12 months due to the secondary effect of grazing on ectomycorrhizal root tips.
近年来，由于其芳香的地下果实，块菌属的物种受到了广泛关注，其在市场上能带来很高的价格。块菌生产的趋势是在温室条件下感染橡树、榛树、山毛榉等。我们旨在展示银冷杉（Abies alba Mill.）是否可以作为商业菌根化的合适宿主伙伴，以及接种基质中的蚯蚓如何影响菌根化动态。分析了接种块菌的银冷杉幼苗的根系参数和菌根化、蚯蚓如何影响裸根系统，以及当蚯蚓被添加到接种基质中时菌根化参数是否会发生变化。在孢子接种后 6 和 12 个月对幼苗进行了分析。有或没有蚯蚓的菌根化对银冷杉幼苗的细根生物量和形态表现出相反的影响。只有少数评估的细根参数表现出统计学上的显著响应，即在接种而无蚯蚓的幼苗中，细根生物量和细根根尖密度较高，接种时添加蚯蚓，根尖密度降低，接种而无蚯蚓的幼苗中特定根尖密度增加，蚯蚓对分枝密度的一般负面影响。银冷杉被确认为与块菌进行商业菌根化的合适宿主伙伴，在接种后 6 个月的菌根化程度为 6%和 35%，在接种后 12 个月的菌根化程度为 36%至 55%。只有在接种后 6 个月，蚯蚓对银冷杉与块菌共生根的影响才是积极的，而在接种后 12 个月，由于蚯蚓对外生菌根根尖的放牧的次生影响，这种影响消失且变得不显著。
