Guillory Roger J, Oliver Alexander A, Davis Emma, Earley Elisha J, Drelich Jaroslaw W, Goldman Jeremy
Department of Biomedical Engineering, Michigan Technological University, USA.
Department of Materials Science and Engineering, Michigan Technological University, USA.
JOM (1989). 2019 Apr;71(4):1436-1446. doi: 10.1007/s11837-019-03371-5. Epub 2019 Feb 12.
Zinc alloy development and characterization for vascular stent application has been facilitated by many standardized and inexpensive methods. In contrast, overly simplistic in vitro approaches dominate the preliminary biological testing of materials. In 2012, our group introduced a metal wire implantation model in rats as a cost effective and realistic approach for the biocompatibility evaluation of degradable materials in the vascular environment. Here, we have adapted metrics routinely used for evaluating stents to quantitatively characterize the long-term progression of the neointima that forms around zinc based wire implants. Histological cross-sections were used to measure the length of neointimal protrusion from the wire into the lumen (denoted wire to lumen thickness), the base neointimal length (describing the breadth of neointimal activation), and the neointimal area. These metrics were used to provide in depth characterization details for neointimal responses to Zn-Mg and Zn-Li alloys and may be used to compare different materials.
许多标准化且成本低廉的方法推动了用于血管支架的锌合金的研发与特性表征。相比之下，过于简单的体外方法主导着材料的初步生物学测试。2012年，我们团队在大鼠中引入了金属丝植入模型，作为一种经济高效且切实可行的方法，用于评估可降解材料在血管环境中的生物相容性。在此，我们采用了常用于评估支架的指标，以定量表征围绕锌基金属丝植入物形成的新生内膜的长期进展情况。组织学横截面用于测量新生内膜从金属丝向管腔突出的长度（表示为金属丝到管腔的厚度）、新生内膜基部长度（描述新生内膜激活的宽度）以及新生内膜面积。这些指标用于提供新生内膜对锌镁合金和锌锂合金反应的深入表征细节，并且可用于比较不同材料。
