Omer Sawsan A, Alsuwaid Duha F, Mohammed Osama B
Department of Zoology, College of Science, King Saud University, University Centre for Women Students, P. O. Box 22452, Riyadh 11495, Saudi Arabia.
Department of Zoology, College of Science, King Saud University, Riyadh 11541, Saudi Arabia.
Saudi J Biol Sci. 2021 Mar;28(3):2023-2028. doi: 10.1016/j.sjbs.2021.01.005. Epub 2021 Jan 15.
The aims of the present study were to characterize ticks infesting the dromedary camel and cattle in Hofuf, Eastern Saudi Arabia and to determine the piroplasms that they may harbor. DNA was extracted from ticks, collected from camels and cattle, using commercial kits and subjected to polymerase chain reaction using specific primers for the amplification of ticks and piroplasms DNA. The cytochrome oxidase subunit I mitochondrial gene () was used for characterization of ticks whereas partial 18S rRNA gene () was used for piroplasms characterization. Ticks were genetically identified as and . Both cattle and camel in Hofuf, were found to be infested with both species. Both ticks identified as and from camels and cows showed 100% identity to sequences from the same species available in GenBank. Only DNA was amplified from both and . infesting cattle. None of the ticks collected from camels revealed DNA of piroplasms. DNA was reported for the first time from Hofuf and the role of both as potential vectors for this parasite in cattle in Saudi Arabia has been documented for the first time.
本研究的目的是鉴定沙特阿拉伯东部胡富夫地区单峰骆驼和牛身上的蜱虫，并确定它们可能携带的梨形虫。使用商业试剂盒从骆驼和牛身上采集的蜱虫中提取DNA，并使用特异性引物进行聚合酶链反应，以扩增蜱虫和梨形虫的DNA。细胞色素氧化酶亚基I线粒体基因（）用于蜱虫的鉴定，而部分18S rRNA基因（）用于梨形虫的鉴定。蜱虫经基因鉴定为和。发现胡富夫地区的牛和骆驼身上均有这两种蜱虫。从骆驼和牛身上鉴定出的和蜱虫与GenBank中同一物种的序列具有100%的同一性。仅从和侵染牛的蜱虫中扩增出了DNA。从骆驼身上采集的蜱虫均未检测到梨形虫的DNA。胡富夫地区首次报告了DNA，并且首次记录了和作为这种寄生虫在沙特阿拉伯牛群中的潜在传播媒介的作用。
