Bardakci Fevzi, Al-Subaie Sarah Hilan Mohammed, Badraoui Riadh, Adnan Mohd, Siddiqui Arif Jamal
Department of Biology, College of Science, University of Ha'il, Ha'il P.O. Box 2440, Saudi Arabia.
Section of Histology-Cytology, Medicine Faculty of Tunis, University of Tunis El Manar, Tunis 1007, Tunisia.
Life (Basel). 2023 Jul 10;13(7):1535. doi: 10.3390/life13071535.
The present study aimed to molecularly identify and characterize the hard ticks infesting camels from the northern region (Ha'il province) of Saudi Arabia using the mitochondrial barcoding gene (). The sequences of tick samples from camels in three regions of Ha'il were aligned with those previously reported from different geographic regions, revealing nine haplotypes, of which six were newly described in this study for the first time. These haplotypes were used to determine their phylogenetic relationships using the maximum likelihood method, displaying two distinct clades corresponding to and . Moreover, the haplotypes showing the highest homology with those deposited in NCBI-GenBank from different geographic regions, including Saudi Arabia, were obtained and combined to determine their phylogenetic relationships among them. The results showed that the haplotypes belonging to two clades were grouped with those previously determined as and . Moreover, the presence of (syn. ) together with suggests possible asymmetrical hybridization and mitochondrial introgression between these species. infesting different mammal species apart from camels were also clustered in a different clade, indicating the presence of different lineages of this species that show different host specificities.
本研究旨在利用线粒体条形码基因对来自沙特阿拉伯北部地区（哈伊勒省）骆驼身上寄生的硬蜱进行分子鉴定和特征分析。将哈伊勒三个地区骆驼身上蜱虫样本的序列与先前报道的来自不同地理区域的序列进行比对，发现了9个单倍型，其中6个是本研究首次新描述的。利用最大似然法，这些单倍型被用于确定它们的系统发育关系，显示出对应于 和 的两个不同分支。此外，获得了与包括沙特阿拉伯在内的不同地理区域保存在NCBI - GenBank中的序列具有最高同源性的单倍型，并将它们组合起来以确定它们之间的系统发育关系。结果表明，属于两个分支的单倍型与先前确定为 和 的单倍型归为一组。此外， （同义词 ）与 的存在表明这些物种之间可能存在不对称杂交和线粒体渐渗。除骆驼外，寄生于不同哺乳动物物种的 也聚集在一个不同的分支中，表明该物种存在显示不同宿主特异性的不同谱系。
