Yu Dan, Liu Haiping, Qin Jianli, Huangfu Mengjie, Guan Xiao, Li Xumei, Zhou Luwei, Dou Tong, Liu Yisa, Wang Lin, Fu Minglei, Wang Juan, Chen Xu
Department of Pharmacy, Guilin Medical University, Guilin, Guangxi Zhuang Autonomous Region 541001, P.R. China.
Science and Technology Department, Guilin Medical University, Guilin, Guangxi Zhuang Autonomous Region 541199, P.R. China.
Oncol Lett. 2021 Apr;21(4):299. doi: 10.3892/ol.2021.12560. Epub 2021 Feb 17.
MicroRNA-30a-5p (miR-30a-5p), which functions as a tumor suppressor, has been reported to be downregulated in colorectal cancer (CRC) tissues and to be associated with cancer invasion. However, the detailed regulatory mechanism of curcumol in the malignant progression of CRC remains unknown. MTT, Transwell, scratch, western blotting and reverse transcription-quantitative PCR assays were performed to examine how curcumol inhibited CRC cell viability, invasion and migration, and to detect the role of miR-30a-5p and curcumol in the invasion and Hippo signaling pathways of CRC cells. The present study revealed that miR-30a-5p expression was downregulated in human CRC tissues and cells. The results demonstrated that miR-30a-5p downregulation was accompanied by the inactivation of the Hippo signaling pathway, which was demonstrated to promote CRC cell viability, invasion and migration. Curcumol treatment was identified to increase miR-30a-5p expression and to activate the Hippo signaling pathway, which in turn inhibited the invasion and migration of CRC cells. Overexpression of miR-30a-5p enhanced the effects of curcumol on cell invasion and migration, and the Hippo signaling pathway in CRC cells. Furthermore, downregulation of miR-30a-5p reversed the effects of curcumol on cell invasion and migration, and the Hippo signaling pathway in CRC cells. These findings identified novel signaling pathways associated with miR-30a-5p and revealed the effects of curcumol on miR-30a-5p expression. Therefore, curcumol may serve as a potential therapeutic strategy to delay CRC progression.
微小RNA-30a-5p（miR-30a-5p）作为一种肿瘤抑制因子，据报道在结直肠癌（CRC）组织中表达下调，并与癌症侵袭相关。然而，莪术醇在CRC恶性进展中的详细调控机制仍不清楚。进行了MTT、Transwell、划痕、蛋白质印迹和逆转录定量PCR分析，以研究莪术醇如何抑制CRC细胞的活力、侵袭和迁移，并检测miR-30a-5p和莪术醇在CRC细胞侵袭和Hippo信号通路中的作用。本研究表明，miR-30a-5p在人CRC组织和细胞中表达下调。结果表明，miR-30a-5p下调伴随着Hippo信号通路的失活，而该信号通路被证明可促进CRC细胞的活力、侵袭和迁移。莪术醇处理可增加miR-30a-5p的表达并激活Hippo信号通路，进而抑制CRC细胞的侵袭和迁移。miR-30a-5p的过表达增强了莪术醇对CRC细胞侵袭、迁移及Hippo信号通路的影响。此外，miR-30a-5p的下调逆转了莪术醇对CRC细胞侵袭、迁移及Hippo信号通路的影响。这些发现确定了与miR-30a-5p相关的新信号通路，并揭示了莪术醇对miR-30a-5p表达 的影响。因此，莪术醇可能是延缓CRC进展的一种潜在治疗策略。
