用于区分耐环丙沙星和敏感鼠伤寒沙门氏菌的基于噬菌体的检测方法的开发。
Development of phage-based assay to differentiate ciprofloxacin resistant and sensitive Typhimurium.
作者信息
Laure Nana Nguefang, Ahn Juhee
机构信息
Department of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Gangwon 24341 Republic of Korea.
出版信息
Food Sci Biotechnol. 2021 Jan 6;30(2):315-320. doi: 10.1007/s10068-020-00858-9. eCollection 2021 Feb.
This study was designed to evaluate the possibility of using phage-amplification assay for discriminating between antibiotic-sensitive and antibiotic-resistant Typhimurium. The characteristics of phage PBST32 were determined by adsorption rate, one-step growth curve, and lytic activity. The ability of phage-based method to detect Typhimurium ATCC 19585 (ST) was determined in single culture and bacterial mixtures of . Typhimurium ATCC 19585 (ST), , and . The adsorption rates of PBST32 were 95% and 93% against ST and ST after 20 min, respectively. The PBST32 showed latent period of 20 min and average burst size of 90 against ST and ST. The ST was selectively detected in mixtures of . , . , and ST by phage amplification assay. These results provide useful information for designing phage amplification method that can differentially detect antibiotic-resistant pathogens.
本研究旨在评估使用噬菌体扩增试验区分抗生素敏感型和耐药型鼠伤寒沙门氏菌的可能性。通过吸附率、一步生长曲线和裂解活性来确定噬菌体PBST32的特性。在鼠伤寒沙门氏菌ATCC 19585（ST）的单培养物以及鼠伤寒沙门氏菌ATCC 19585（ST）、[此处原文缺失部分内容]和[此处原文缺失部分内容]的细菌混合物中，测定基于噬菌体的方法检测鼠伤寒沙门氏菌ATCC 19585（ST）的能力。20分钟后，PBST32对ST和ST的吸附率分别为95%和93%。PBST32对ST和ST的潜伏期为20分钟，平均裂解量为90。通过噬菌体扩增试验在[此处原文缺失部分内容]、[此处原文缺失部分内容]和ST的混合物中选择性地检测到了ST。这些结果为设计能够差异检测耐药病原体的噬菌体扩增方法提供了有用信息。
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