Basic Medicine College, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Department of Pediatrics, The Second Hospital Affiliated Shaanxi University of Chinese Medicine, Shaanxi, China.
Front Public Health. 2021 Mar 1;9:650022. doi: 10.3389/fpubh.2021.650022. eCollection 2021.
The herbs and (RCRS) are often used in traditional Chinese medicine for the treatment of uterine leiomyoma (UL). The effectiveness of RCRS for the treatment of UL has been confirmed in our previous studies. This study aimed to investigate the molecular mechanism by which RCRS inhibits the activation of fibroblast activation protein (FAP) and prevents UL in rats. A Sprague Dawley (SD) rat model of UL was established via estrogen and progesterone load combined with external stimulation. Histological analyses, enzyme-linked immunosorbent assays, and western blotting were performed to evaluate the effect of RCRS on UL and elucidate its mechanism of action. Our data showed that the treatment of SD rats with RCRS significantly reduced the expression of extracellular matrix component collagen, FAP, and transforming growth factor beta (a FAP-activating factor) and the phosphorylation of the cell proliferation pathway-related signaling factors AKT/MEK/ERK. Our results suggest that RCRS is effective in the prevention and treatment of UL in rats, and RCRS may exert its functions by inhibiting the activation of tumor-associated fibroblasts and cell proliferation and by improving the tumor extracellular matrix.
该草药和（RCRS）常用于传统中药治疗子宫肌瘤（UL）。我们之前的研究已经证实了 RCRS 治疗 UL 的有效性。本研究旨在探讨 RCRS 抑制成纤维细胞激活蛋白（FAP）激活并预防大鼠 UL 的分子机制。通过雌激素和孕激素负荷联合外部刺激建立了 Sprague Dawley（SD）大鼠 UL 模型。通过组织学分析、酶联免疫吸附试验和 Western blot 评估 RCRS 对 UL 的影响并阐明其作用机制。我们的数据表明，用 RCRS 治疗 SD 大鼠可显著降低细胞外基质成分胶原、FAP 和转化生长因子β（FAP 激活因子）的表达以及与细胞增殖途径相关的信号因子 AKT/MEK/ERK 的磷酸化。我们的结果表明，RCRS 可有效预防和治疗大鼠 UL，RCRS 可能通过抑制肿瘤相关成纤维细胞和细胞增殖的激活以及改善肿瘤细胞外基质来发挥作用。
