Ceanga Mihai, Guenther Madlen, Ingrisch Ina, Kunze Albrecht
Hans Berger Department of Neurology, Jena University Hospital, Am Klinikum， Jena, Germany.
Bio Protoc. 2021 Jan 20;11(2):e3890. doi: 10.21769/BioProtoc.3890.
Long-term consequences of stroke significantly impair the quality of life in a growing population of stroke survivors. Hippocampal adult neurogenesis has been hypothesized to play a role in the pathophysiology of cognitive and neuropsychiatric long-term sequelae of stroke. Reliable animal models of stroke are paramount to understanding their biomechanisms and to advancing therapeutic strategies. We present a detailed protocol of a transient cerebral ischemia model which does not cause direct ischemic damage in the hippocampus, allowing investigations into the pathophysiology of long-term neurocognitive deficits of stroke. Furthermore, we describe a protocol for obtaining acute hippocampal slices for the purpose of electrophysiological and morphological characterization of adult-borne granule cells. Particularities relating to performing electrophysiological recordings from small cells, such as immature adult-borne granule cells, are also discussed. The present protocol may be complemented by multi-modal investigations (behavioral, morpho-structural, biochemical), to hopefully facilitate research and advances into the long-term sequelae of stroke and the discovery of new therapeutic opportunities.
中风的长期后果严重损害了越来越多中风幸存者的生活质量。海马体成年神经发生被认为在中风的认知和神经精神长期后遗症的病理生理学中发挥作用。可靠的中风动物模型对于理解其生物机制和推进治疗策略至关重要。我们提出了一种短暂性脑缺血模型的详细方案，该模型不会在海马体中造成直接缺血损伤，从而能够研究中风长期神经认知缺陷的病理生理学。此外，我们描述了一种获取急性海马切片的方案，用于对成年新生颗粒细胞进行电生理和形态学特征分析。还讨论了从小细胞（如未成熟的成年新生颗粒细胞）进行电生理记录的相关特殊性。本方案可通过多模态研究（行为学、形态结构学、生物化学）加以补充，有望促进对中风长期后遗症的研究和进展，并发现新的治疗机会。
