Sheetz M P
Prog Clin Biol Res. 1977;17:559-67.
We have found that human erythrocyte ghosts in 10 mM HEPES (pH 7.0) at 0 degrees C would crenate when 20-50 mM of Na+ or K+, 0.2-0.5 mM OF Ca++, Ba++, Sr++, or Mg++, or 10 muM of La+++ was added. The shape change after cation addition was faster than fixation by 1% glutaraldehyde at 4 degrees C and was readily reversible upon dilution of the cation. After incubation of ghosts in 10 mM HEPES (pH 7.0) at 37 degrees for 10-20 min there was a significant inhibition of subsequent crenation by cations. In a process that is believed to occur by a similar mechanism, whole red blood cells were observed to cup (invaginate) when 20 mM of a divalent or 0.1 mM of a trivalent cation was added. After neuraminidase treatment to remove the sialic acid charge groups, these same shape changes were observed in ghosts and whole cells. Another type of cation-induced crenation was found to follow upon the addition to whole cells of A23187 and Ca++ or Ba++ but not Mg++. This process is much slower than crenation in the ghost and is believed to be caused by a different mechanism.
我们发现，在0℃下，将人红细胞血影置于10 mM HEPES（pH 7.0）中，当加入20 - 50 mM的Na⁺或K⁺、0.2 - 0.5 mM的Ca²⁺、Ba²⁺、Sr²⁺或Mg²⁺，或10 μM的La³⁺时，血影会发生皱缩。加入阳离子后形状的变化比在4℃下用1%戊二醛固定更快，并且在稀释阳离子后形状很容易恢复。将血影在37℃的10 mM HEPES（pH 7.0）中孵育10 - 20分钟后，阳离子随后引起的皱缩受到显著抑制。在一个被认为通过类似机制发生的过程中，当加入20 mM的二价阳离子或0.1 mM的三价阳离子时，观察到完整红细胞会形成杯状（内陷）。用神经氨酸酶处理以去除唾液酸电荷基团后，在血影和完整细胞中观察到相同的形状变化。发现另一种类型的阳离子诱导皱缩是在向完整细胞中加入A23187和Ca²⁺或Ba²⁺而不是Mg²⁺后发生的。这个过程比血影中的皱缩慢得多，并且被认为是由不同的机制引起的。
