Yasuda Kensuke, Kubo Yuki, Murata Hiroki, Sakamoto Kazuichi
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan.
Biochem Biophys Rep. 2021 Mar 8;26:100961. doi: 10.1016/j.bbrep.2021.100961. eCollection 2021 Jul.
In this study, we studied the effects of cortisol and cortisone on the age-related decrease in locomotion in the nematode and on the tolerance to heat stress at 35 °C and to oxidative stress induced by the exposure to 0.1% HO. Changes in mRNA expression levels of genes related to stress tolerance were also analyzed. Cortisol treatment restored nematode movement following heat stress and increased viability under oxidative stress, but also shortened worm lifespan. Cortisone, a cortisol precursor, also restored movement after heat stress. Additionally, cortisol treatment increased mRNA expression of the and genes. Furthermore, cortisol treatment failed to restore movement of -deficient mutants after heat stress, whereas cortisone failed to restore the movement of -deficient mutants after heat stress. In conclusion, the results suggested that cortisol promoted stress tolerance via DAF-16 but shortened the lifespan, whereas cortisone promoted stress tolerance via DHS-30.
在本研究中，我们研究了皮质醇和可的松对线虫运动能力随年龄下降的影响，以及对35°C热应激和暴露于0.1%过氧化氢诱导的氧化应激的耐受性。还分析了与应激耐受性相关基因的mRNA表达水平变化。皮质醇处理可恢复热应激后线虫的运动，并提高氧化应激下的活力，但也缩短了线虫寿命。可的松作为皮质醇的前体，也能在热应激后恢复运动。此外，皮质醇处理增加了和基因的mRNA表达。此外，皮质醇处理未能恢复热应激后缺陷突变体的运动，而可的松未能恢复热应激后缺陷突变体的运动。总之，结果表明皮质醇通过DAF-16促进应激耐受性，但缩短了寿命，而可的松通过DHS-30促进应激耐受性。
