Vesnina Anna, Milentyeva Irina, Minina Varvara, Kozlova Oksana, Asyakina Lyudmila
Laboratory of Natural Nutraceuticals Biotesting, Research Department, Kemerovo State University, 650043 Kemerovo, Russia.
Department of Genetic and Fundamental Medicine, Kemerovo State University, 650000 Kemerovo, Russia.
Life (Basel). 2023 Aug 8;13(8):1706. doi: 10.3390/life13081706.
This study aimed to investigate the anti-atherosclerotic properties of quercetin isolated from the extract of Ledeb hairy roots. During the study, the hormonal composition of the nutrient medium for cultivation of hairy root biomass was selected: Gamborg's medium enriched with the cytokine 6-benzylaminopurine (1.5 mg/1 dm). It was found that the extraction of hairy root biomass with a 50% water-ethanol solution (40:1 1 h at 60 ± 2 °C) yielded an extract that contained the highest amount of quercetin (an average of 2.1 times higher than in extracts obtained at other parameters). It was determined that 100 µM quercetin solution showed the greatest bioactivity on : on day 61, the percentage of surviving nematodes was 2.06 times higher compared to other samples and 6 times higher compared to control, resulting in a 12.5-fold increase in expression compared to control (without biologically active substance (BAS) addition). Meanwhile, the 10 µM quercetin solution exhibited the best ability to inhibit the accumulation of lipid fractions; the accumulation was 1.06 times less compared to the control. The results of this study show that quercetin, which was isolated from the biomass of hairy roots, can be used as a component of anti-atherosclerotic dietary supplements.
本研究旨在探究从细叶益母草毛状根提取物中分离得到的槲皮素的抗动脉粥样硬化特性。在研究过程中，选择了用于培养毛状根生物量的营养培养基的激素组成：富含细胞分裂素6-苄氨基嘌呤（1.5毫克/1立方分米）的甘博格培养基。结果发现，用50%水-乙醇溶液（40:1，在60±2℃下提取1小时）提取毛状根生物量，得到的提取物中槲皮素含量最高（平均比在其他参数下获得的提取物高2.1倍）。已确定100微摩尔槲皮素溶液表现出最大的生物活性：在第61天，存活线虫的百分比比其他样品高2.06倍，比对照高6倍，与对照（未添加生物活性物质）相比，表达增加了12.5倍。同时，10微摩尔槲皮素溶液表现出最佳的抑制脂质组分积累的能力；与对照相比，积累量减少了1.06倍。本研究结果表明，从毛状根生物量中分离得到的槲皮素可作为抗动脉粥样硬化膳食补充剂的一种成分。
