Maeyama Hiroki, Shinmyo Yohei, Kawasaki Hiroshi
Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Ishikawa, 920-8640, Japan.
Biochem Biophys Rep. 2021 Mar 6;26:100970. doi: 10.1016/j.bbrep.2021.100970. eCollection 2021 Jul.
Aristaless-related homeobox (ARX) has important functions in the development of various organs including the brain. Mutations of the human gene have been associated with malformations of the cerebral cortex such as microcephaly and lissencephaly. Although the expression patterns of ARX in the lissencephalic cerebral cortex of mice have been intensively investigated, those in expanded gyrencephalic brains remained unclear. Here, we show the expression patterns of ARX in the developing cerebral cortex of gyrencephalic carnivore ferrets. We found that ARX was expressed not only in intermediate progenitor (IP) cells but also in outer radial glial (oRG) cells, which are neural progenitors preferentially observed in the gyrencephalic cerebral cortex. We found that the majority of ARX-positive oRG cells expressed the proliferating cell marker Ki-67. These results may indicate that ARX in oRG cells mediates the expansion of the gyrencephalic cerebral cortex during development and evolution.
无尾相关同源框（ARX）在包括大脑在内的各种器官发育中具有重要功能。人类该基因的突变与大脑皮质畸形如小头畸形和无脑回畸形有关。尽管已经对小鼠无脑回大脑皮质中ARX的表达模式进行了深入研究，但在脑回扩展的大脑中其表达模式仍不清楚。在此，我们展示了ARX在脑回发达的食肉动物雪貂发育中的大脑皮质中的表达模式。我们发现ARX不仅在中间祖细胞（IP）中表达，还在外侧放射状胶质细胞（oRG）中表达，oRG细胞是在脑回发达的大脑皮质中优先观察到的神经祖细胞。我们发现大多数ARX阳性oRG细胞表达增殖细胞标记物Ki-67。这些结果可能表明oRG细胞中的ARX在发育和进化过程中介导了脑回发达的大脑皮质的扩展。
