Département des Sciences Cliniques des animaux de compagnie et de sport, École nationale vétérinaire de Toulouse, Université de Toulouse, INSERM, ENVT, UPS, Toulouse, France.
CREFRE, École nationale vétérinaire de Toulouse, Université de Toulouse, INSERM, ENVT, UPS, Toulouse, France.
J Vet Diagn Invest. 2021 May;33(3):572-576. doi: 10.1177/10406387211001092. Epub 2021 Mar 18.
causes life-threatening heart disease in dogs, thus screening of dog populations is important. Lens-free technology (LFT) is a low-cost imaging technique based on light diffraction that allows computerized recognition of small objects in holographic images. We evaluated an algorithm capable of recognizing microfilariae in canine whole blood using the LFT. We examined 3 groups of 10 EDTA blood specimens, from dogs with microfilaremia (group A), healthy dogs (B), and dogs with hematologic modifications other than microfilaremia (C). The LFT analyzer photographed repeated series of 5 images of all samples. The algorithm declared a sample positive if a microfilaria was detected on ≥1, ≥2, or ≥3 of the 5 images of a series. Microfilariae were detected visually in the images in 9 of 10 cases in group A; no microfilariae were seen in the images from groups B and C. Of the 30 cases, there were 14, 4, and only 3 false-positives with the 1 of 5, 2 of 5, and 3 of 5 image cutoffs, respectively. There were no false-negatives, regardless of cutoff. LFT seems useful for detecting microfilaria and could have application in clinical pathology.
犬恶丝虫可导致危及生命的心脏病，因此对犬群进行筛查很重要。无透镜技术（LFT）是一种基于光衍射的低成本成像技术，可实现计算机识别全息图像中的小物体。我们评估了一种使用 LFT 识别犬全血中微丝蚴的算法。我们检查了来自微丝蚴血症犬（A 组）、健康犬（B 组）和除微丝蚴血症外血液学改变的犬（C 组）的 3 组每组 10 个 EDTA 血标本。LFT 分析仪对所有样本的 5 张重复图像系列进行拍照。如果在一个系列的 5 张图像中的≥1、≥2 或≥3 张图像上检测到微丝蚴，则算法宣布该样本为阳性。在 A 组的 10 个病例中的 9 个中，在图像中可肉眼观察到微丝蚴；在 B 组和 C 组的图像中未观察到微丝蚴。在 30 个病例中，1 个、2 个和 3 个微丝蚴的 5 个图像截止值分别为 14、4 和仅 3 个假阳性；无论截止值如何，均无假阴性。LFT 似乎可用于检测微丝蚴，并且可能在临床病理学中有应用。
