Xie Jiaojiao, Yao Mingfei, Lu Yanmeng, Yu Mengjia, Han Shengyi, McClements David J, Xiao Hang, Li Lanjuan
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310003, China.
Food Funct. 2021 Apr 7;12(7):3180-3190. doi: 10.1039/d0fo03235b. Epub 2021 Mar 18.
Antibiotic treatment is often followed by Clostridium difficile infection (CDI), which causes severe diarrhea and other health issues. Oral administration of Pediococcus pentosaceus Li05 (Li05) has been shown to have great potential in preventing CDI. However, the viability of Li05 is greatly reduced during storage and passage through the gastrointestinal (GI) tract, which limits its biological activity. In this study, a gastro-responsive microgel was designed to encapsulate and protect Li05 to enhance its efficacy against CDI. The viability of Li05 encapsulated within the microgels was significantly enhanced during long-term storage and after exposure to simulated GI fluids. Moreover, this gastro-responsive microgel led to greater sustained release of the probiotic. In a mouse CDI model, we found that encapsulated Li05 was better at inhibiting C. difficile infection than nonencapsulated Li05, as demonstrated through analysis of the probiotic survival rate, spleen weight, colonic histology, and inflammatory cytokine levels. Moreover, the gut microbial diversity was enriched by treatment with encapsulated Li05. These results suggest that encapsulating Li05 within biopolymer microgels may enhance its ability to prevent and treat CDI using functional foods, supplements, or pharmaceuticals.
抗生素治疗后常继发艰难梭菌感染（CDI），该感染会导致严重腹泻及其他健康问题。已证明口服戊糖片球菌Li05（Li05）在预防CDI方面具有巨大潜力。然而，Li05在储存及通过胃肠道（GI）时活力会大幅降低，这限制了其生物活性。在本研究中，设计了一种胃响应性微凝胶来包裹并保护Li05，以增强其抗CDI的功效。包裹在微凝胶中的Li05在长期储存期间以及暴露于模拟胃肠液后活力显著增强。此外，这种胃响应性微凝胶能使益生菌实现更大程度的持续释放。在小鼠CDI模型中，通过分析益生菌存活率、脾脏重量、结肠组织学及炎性细胞因子水平发现，包裹的Li05在抑制艰难梭菌感染方面比未包裹的Li05效果更好。此外，用包裹的Li05进行治疗可丰富肠道微生物多样性。这些结果表明，将Li05包裹在生物聚合物微凝胶中可能会增强其利用功能性食品、补充剂或药物预防和治疗CDI的能力。
