State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
Food Funct. 2021 Jun 7;12(11):5077-5086. doi: 10.1039/d0fo02530e. Epub 2021 May 7.
Many Pediococcus spp. have health-promoting benefits, and Pediococcus pentosaceus LI05 is one such species that was proved to be beneficial in previous studies. Our research aimed to determine the immune and metabolic effects of P. pentosaceus LI05 on germ-free rats. Germ-free rats were gavaged with P. pentosaceus LI05 suspensions (1 × 10 CFU) for 2 weeks, and 3 weeks later, blood, spleen, intestine and liver samples were gathered for metabolome, intestine morphology, immunity, and transcriptomics analyses. Oral gavage of P. pentosaceus LI05 reduced the bodyweight of rats, which manifested as increased fecal carbohydrate concentrations, decreased intestinal fat intake and the hepatic fat synthesis gene expression, and accelerated fat-to-glycogen conversion. In addition, P. pentosaceus LI05 exhibited an anti-inflammatory ability, reducing serum proinflammatory cytokine levels and increasing intestinal subepidermal CD4 cell levels. Furthermore, administration of P. pentosaceus LI05 increased the antimicrobial ability and enhanced the liver detoxification function. These results indicate that as a probiotic, P. pentosaceus LI05 ameliorates the hampered immune response of GF animals and improves the metabolism of fat and toxic substances.
许多肠球菌属（Pediococcus spp.）具有促进健康的益处，戊糖片球菌（Pediococcus pentosaceus）LI05 就是这样一种在之前的研究中被证明有益的物种。我们的研究旨在确定戊糖片球菌 LI05 对无菌大鼠的免疫和代谢影响。无菌大鼠用戊糖片球菌 LI05 悬浮液（1×10CFU）灌胃 2 周，3 周后采集血液、脾脏、肠道和肝脏样本进行代谢组学、肠道形态学、免疫和转录组学分析。口服戊糖片球菌 LI05 降低了大鼠的体重，表现为粪便碳水化合物浓度增加，肠道脂肪摄入减少和肝脂肪合成基因表达减少，以及加速脂肪向肝糖原的转化。此外，戊糖片球菌 LI05 表现出抗炎能力，降低血清促炎细胞因子水平，增加肠道表皮下 CD4 细胞水平。此外，给予戊糖片球菌 LI05 增加了抗菌能力，并增强了肝脏解毒功能。这些结果表明，作为一种益生菌，戊糖片球菌 LI05 改善了 GF 动物受损的免疫反应，并改善了脂肪和有毒物质的代谢。
