Department of Crop Sciences, University of Illinois, Urbana, IL 61801.
Phytopathology. 2021 Aug;111(8):1289-1300. doi: 10.1094/PHYTO-06-20-0228-R. Epub 2021 Sep 11.
Bacterial leaf spot disease caused by has severely affected the pumpkin industries in the Midwestern region of United States, with the bacteria mainly infecting pumpkin leaves and fruits, and leading to significant yield losses. In this study, we utilized genomics and genetics approaches to elucidate molecular mechanisms of pathogenesis during interaction with its host. We generated the first reference-quality whole-genome sequence of the type isolate and compared with other species, has a smaller genome size with fewer virulence-related genes. RNA-seq analysis of under plant-mimicking media conditions showed altered transcriptional responses, with upregulation of virulence genes and downregulation of cellular homeostasis genes. Additionally, characterization of key virulence genes using gene deletion methods revealed that both type II enzymes and type III effectors are necessary for to cause infection in the pumpkin host.
由 引起的细菌性叶斑病严重影响了美国中西部地区的南瓜产业，这种细菌主要感染南瓜的叶片和果实，导致产量显著下降。在这项研究中，我们利用基因组学和遗传学方法来阐明与宿主相互作用过程中的致病分子机制。我们生成了 模式分离株的第一个参考质量的全基因组序列，并与其他 物种进行了比较，结果表明 具有较小的基因组大小和较少的毒力相关基因。在植物模拟培养基条件下对 进行 RNA-seq 分析显示，转录应答发生改变，毒力基因上调，细胞内稳态基因下调。此外，利用基因缺失方法对关键毒力基因进行表征发现，Ⅱ型酶和Ⅲ型效应子对于 在南瓜宿主中引起感染都是必要的。
