印度南部 2 型糖尿病患者维生素 D 受体（、和）基因多态性与维生素 D 和血糖状态的关系。

Association of genetic polymorphisms in vitamin D receptor (, and ) with vitamin D and glycemic status in type 2 diabetes patients from Southern India.

机构信息

Department of Clinical Pharmacology, JIPMER, Puducherry, India.

Department of Medicine, JIPMER, Puducherry, India.

出版信息

Drug Metab Pers Ther. 2021 Mar 18;36(3):183-187. doi: 10.1515/dmpt-2020-0178.

DOI:10.1515/dmpt-2020-0178

PMID:33735953

Abstract

OBJECTIVES

To evaluate the association of polymorphisms (, and ) with vitamin D levels and glycemic status in type 2 diabetes patients from Southern India.

METHODS

In this observational study, genotype frequencies and vitamin D levels of 200 cases (type 2 diabetes patients) were compared with 300 controls (unrelated anonymised stored samples of healthy volunteers) from south India. Serum 25 (OH) D levels were measured by immunoassay technique, glycated hemoglobin (HbA) was measured using HPLC and genotyping of VDR polymorphisms were carried out using Real time Polymerase Chain Reaction (RT PCR).

RESULTS

About 69.2% of type 2 diabetes patients were found to have vitamin D deficiency. polymorphism showed variations in serum 25 (OH) D levels, with AA and AG genotypes having significantly lower serum 25 (OH) D levels as compared to GG [13.24 (8.4) ng/ml, 15.02 (7.07) ng/ml and 20.67 (13.64) ng/ml respectively]. There was no difference in HbA levels with respect to the vitamin D levels and VDR polymorphisms.

CONCLUSIONS

AA and AG genotypes of polymorphisms are associated with low serum 25 (OH) D levels. However there was no association between VDR polymorphisms and glycemic status in south Indian type 2 diabetes patients.

摘要

目的

评估、和多态性与印度南部 2 型糖尿病患者维生素 D 水平和血糖状态的关联。

方法

在这项观察性研究中，比较了来自印度南部的 200 例（2 型糖尿病患者）病例和 300 例对照（无关的匿名健康志愿者储存样本）的基因型频率和维生素 D 水平。采用免疫测定技术检测血清 25（OH）D 水平，采用高效液相色谱法检测糖化血红蛋白（HbA），采用实时聚合酶链反应（RT-PCR）进行 VDR 多态性基因分型。

结果

约 69.2%的 2 型糖尿病患者存在维生素 D 缺乏。多态性与血清 25（OH）D 水平存在差异，与 GG 基因型相比，AA 和 AG 基因型的血清 25（OH）D 水平显著降低[分别为 13.24（8.4）ng/ml、15.02（7.07）ng/ml 和 20.67（13.64）ng/ml]。维生素 D 水平和 VDR 多态性与 HbA 水平无差异。