School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, PR China.
Experimental Center, Shandong University of Traditional Chinese Medicine, Jinan 250355, PR China.
Int J Biol Macromol. 2020 Dec 15;165(Pt B):2621-2629. doi: 10.1016/j.ijbiomac.2020.10.163. Epub 2020 Oct 25.
Many effects of Coprinus comatus are attributed to its polysaccharide components. Therefore, the aim of this article is to take Coprinus comatus polysaccharides as the research topic to estimate the difference between the polysaccharides of Coprinus comatus fruiting bodies (CBPs) and the intracellular polysaccharides of liquid fermentation (ICPs). The total carbohydrate contents, monosaccharide compositions, molecular weights, functional groups, microstructures and functional properties of the two prepared polysaccharides were evaluated. At the same time, the influences of the two polysaccharides on the proliferation of lactobacillus and Bifidobacterium in vitro were compared. The structural analysis exhibited that there were slight differences in the two prepared polysaccharides. However, both ICPs and CBPs could be utilized by these two strains. Furthermore, the effects of the two prepared polysaccharides on the proliferation of the selected probiotics were dose-dependent manners within the scope of the experiment, and the ICPs group and CBPs group had no significant difference (P > 0.05). Therefore, this work demonstrates that ICPs could be an equivalent replacer for CBPs.
许多鸡腿菇的作用都归因于其多糖成分。因此，本文旨在以鸡腿菇多糖为研究课题，估算鸡腿菇子实体多糖（CBPs）和液体发酵胞内多糖（ICPs）之间的差异。评估了两种制备多糖的总碳水化合物含量、单糖组成、分子量、官能团、微观结构和功能特性。同时，比较了两种多糖对体外乳杆菌和双歧杆菌增殖的影响。结构分析表明，两种制备的多糖略有差异。然而，两种菌株都可以利用 ICPs 和 CBPs。此外，两种制备的多糖对所选益生菌增殖的影响呈剂量依赖性，ICPs 组和 CBPs 组之间无显著性差异（P＞0.05）。因此，这项工作表明 ICPs 可以作为 CBPs 的等效替代品。
