金鱼红鲫鱼 GATA1 基因的克隆与表达分析
Cloning and expression analysis of GATA1 gene in Carassius auratus red var.
机构信息
Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, School of Life Sciences, Hunan University of Science and Technology, Xiangtan, 411201, Hunan, People's Republic of China.
Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, Guangdong, Hunan, People's Republic of China.
出版信息
BMC Genom Data. 2021 Mar 18;22(1):12. doi: 10.1186/s12863-021-00966-3.
BACKGROUND
GATA1 is a key transcription factor in the GATA family, and promotes the differentiation and maturation of red blood cell, which is essential for normal hematopoiesis.
RESULTS
Our results showed that the cDNA sequence of GATA1 was 2730 bp long encoding 443 amino acids. qRT-PCR analysis demonstrated that GATA1 had the highest expression in testis (T), followed by pituitary (P) and spleen (S). GATA1 gene expression in C. auratus red var. embryo from the neuroblast stage (N) to the embryo hatching (H) changes continuously; and the gene expression levels of nonylphenol (NP)-treated and those of control embryos were significantly different. Moreover, Methylation levels of GATA1 gene in NP-treated embryos were higher than those in control embryos, indicating that NP affected GATA1 methylation.
CONCLUSIONS
Our study provides cues for further studying the roles of GATA1 gene in fish development, and suggested a potential molecular mechanism by which NP leads to abnormal development of fish embryos.
背景
GATA1 是 GATA 家族中的关键转录因子，可促进红细胞的分化和成熟，这对于正常的造血至关重要。
结果
我们的结果表明，GATA1 的 cDNA 序列长 2730bp，编码 443 个氨基酸。qRT-PCR 分析表明，GATA1 在睾丸（T）中的表达最高，其次是垂体（P）和脾脏（S）。GATA1 基因在草鱼胚胎从神经胚期（N）到孵化（H）的连续变化中表达；并且壬基酚（NP）处理的胚胎和对照胚胎的基因表达水平有显著差异。此外，NP 处理的胚胎中 GATA1 基因的甲基化水平高于对照胚胎，表明 NP 影响 GATA1 甲基化。
结论
本研究为进一步研究 GATA1 基因在鱼类发育中的作用提供了线索，并提出了 NP 导致鱼类胚胎发育异常的潜在分子机制。
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