Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100, Burjassot, València, Spain.
Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100, Burjassot, València, Spain.
Food Chem Toxicol. 2021 May;151:112129. doi: 10.1016/j.fct.2021.112129. Epub 2021 Mar 16.
Cytoprotection effects of Allium sativum L garlic extract from a local garlic ecotype from Ferrara (Italy) on hepatocarcinoma cells, HepG2 cells, is presented in this study. This garlic type is known as Voghiera garlic and has been characterized as PDO (Protected designation of Origin) product. Voghiera garlic extract (VGE) was evaluated against beauvericin (BEA) and two zearalenone (ZEA) metabolites (α-zearalenol (α-ZEL) and β-zearalenol (β-ZEL))-induced cytotoxicity on HepG2 cells by the MTT (3-4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, over 24 h and 48 h. Direct treatment, simultaneous treatment and pre-treatment strategies at the dilution 1:16-1:00 for VGE and at the concentration range from 0.08 to 2.5 μM for BEA and from 1.6 to 50 μM for both α-ZEL and β-ZEL were tested. Individual IC values were detected at all times assayed for BEA (>0.75 μM) and VGE (dilution upper 1:8) while this was not observed for ZEA's metabolites. When simultaneous strategy of VGE + mycotoxin was tested, cytoprotection with increases of viability (upper 50%) were observed. Lastly, in pre-treatment strategy with VGE, viability of HepG2 cells was significantly protected when α-ZEL was tested. As a result, the greatest cytoprotective effect of VGE in HepG2 cells is obtained when simultaneous treatment strategy was performed.
本研究介绍了来自意大利费拉拉（意大利）当地大蒜生态型的大蒜提取物（大蒜）对肝癌细胞（HepG2 细胞）的细胞保护作用。这种大蒜类型被称为 Voghiera 大蒜，已被确认为 PDO（受保护的原产地名称）产品。通过 MTT（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴盐）测定法，评估了 Voghiera 大蒜提取物（VGE）对 beauvericin（BEA）和两种玉米赤霉烯酮（ZEA）代谢物（α-zearalenol（α-ZEL）和β-zearalenol（β-ZEL））在 HepG2 细胞上的细胞毒性，作用时间为 24 小时和 48 小时。直接处理、同时处理和预处理策略在 VGE 的稀释度 1:16-1:00 和 BEA 的浓度范围从 0.08 到 2.5μM 以及 α-ZEL 和 β-ZEL 的浓度范围从 1.6 到 50μM 进行了测试。在所有时间点检测到 BEA（>0.75μM）和 VGE（稀释度最高 1:8）的个体 IC 值，但未观察到 ZEA 代谢物的 IC 值。当同时测试 VGE+真菌毒素的策略时，观察到细胞活力（最高 50%）增加的细胞保护作用。最后，在 VGE 的预处理策略中，当测试 α-ZEL 时，HepG2 细胞的活力得到了显著保护。因此，当同时进行治疗策略时，VGE 对 HepG2 细胞的最大细胞保护作用。
