Department of Pharmacognosy and Natural Medicine, Faculty of Pharmacy, University of Uyo, Uyo, Nigeria.
Department of Pharmacognosy and Natural Medicine, Faculty of Pharmacy, University of Uyo, Uyo, Nigeria.
J Ethnopharmacol. 2021 Nov 15;280:114031. doi: 10.1016/j.jep.2021.114031. Epub 2021 Mar 15.
Hippocratea africana root is used in African folk medicine for the treatment of several ailments, including pain and inflammation.
To isolate anti-inflammatory and analgesic compounds from the roots of H. africana, with accompanying antioxidant potentials.
Dichloromethane, ethyl acetate, and aqueous fractions of H. africana roots, and isolated compounds from the bioactive ethyl acetate fraction were evaluated for anti-inflammatory and analgesic activities using the xylene induced oedema in mice and thermal induced pain models, respectively. The antioxidant potentials of isolated compounds were tested in 2,2-diphenyl-1-picryhydrazyl radical and ferric reducing antioxidant power assays. Structures were elucidated on the basis of spectroscopic analyses, including 1D and 2D NMR experiments, ionization mass spectrometry, and comparison with literature data.
Isoathyriol (1,3,7-trihydroxy-6-methoxyxanthone) and norathyriol (1,3,6,7-tetrahydroxyxanthone) were isolated from the potent anti-inflammatory and analgesic ethyl acetate fraction of H. africana roots. Isoathyriol and norathyriol demonstrated good anti-inflammatory, analgesic, and antioxidant properties compared with the standards used in each assay.
This study substantiates the use of H. africana root extract in the alleviation of inflammation and pain, and reports the characterization of secondary metabolites in H. africana and for the first time the presence of xanthones in Hippocratea genus.
非洲 Hippocratea 根在非洲民间医学中用于治疗多种疾病，包括疼痛和炎症。
从非洲 Hippocratea 根中分离具有抗炎和镇痛作用的化合物，并伴随抗氧化潜力。
用二甲亚砜、乙酸乙酯和水层对非洲 Hippocratea 根进行处理，用二甲苯诱导的小鼠水肿模型和热诱导疼痛模型分别评估生物活性乙酸乙酯部分的抗炎和镇痛活性。用 2,2-二苯基-1-苦肼基自由基和铁还原抗氧化能力测定法测试分离化合物的抗氧化潜力。根据光谱分析，包括 1D 和 2D NMR 实验、离子质谱和与文献数据的比较，确定结构。
从具有强大抗炎和镇痛活性的非洲 Hippocratea 根的乙酸乙酯部分中分离出异桉叶醇（1,3,7-三羟基-6-甲氧基黄烷酮）和去甲桉叶醇（1,3,6,7-四羟基黄烷酮）。与每个测定中使用的标准相比，异桉叶醇和去甲桉叶醇表现出良好的抗炎、镇痛和抗氧化特性。
本研究证实了非洲 Hippocratea 根提取物在缓解炎症和疼痛方面的用途，并报告了 Hippocratea 属中次生代谢物的特征以及黄烷酮类化合物在 Hippocratea 属中的首次出现。
