Department of Conservative Dental Sciences, College of Dentistry, Prince Sattam Bin Abdulaziz University, P.O. BOX: 173, Al-Kharj, 11942, Saudi Arabia.
Department of Restorative Dentistry, Endodontics Division, College of Dentistry, Najran University, P.O Box 1988, Najran, 66446, Saudi Arabia.
Photodiagnosis Photodyn Ther. 2021 Jun;34:102257. doi: 10.1016/j.pdpdt.2021.102257. Epub 2021 Mar 15.
To investigate the efficacy of conventional root canal treatment (cRCT) with adjunctive photodynamic therapy (aPDT) against microbial biofilms within infected c-shaped root canals.
In this in vitro report, the inoculation of 20 freshly extracted human mandibular molar teeth having c-shaped root canal configuration was performed with E. faecalis and P. aeruginosa to produce three-day biofilms in prepared canal system. PDT used a combination of chlorin (ce6) and polyethylenimine (PEI) as the photosensitizer (PS). A 200 μ-fiber was employed to deliver a 660 nm diode laser light into the root canal, and this was compared and conjugated with conventional endodontic treatment utilizing antiseptic irrigation and mechanical debridement.
The utilization of aPDT (group-2) resulted in a considerable decrease in the count of E. faecalis and P. aeruginosa from 12.84 ± 2.18 CFU/mL to 5.13 ± 0.67 CFU/mL, and from 14.06 ± 3.98 CFU/mL to 4.82 ± 1.05 CFU/mL pre-and post-treatment, respectively. A statistically significant reduction in the bacterial counts of both microbes was observed after treatment among the samples of the both study groups (p < 0.05). Specimens in group-2 (8.42 ± 1.14 MPa) demonstrated the highest mean push-out bond strength, whereas the lowest was shown by samples in group-1 (7.08 ± 1.09 MPa). ANOVA showed no statistical difference between the research groups (p = 0.676). The independent t-test revealed that the mean push-out bond strength scores of the cervical segments were higher than the apical and middle segments of roots in research groups (p < 0.05).
In c-shaped root canals, the application of photodynamic therapy as an adjuvant to conventional root canal treatment contributes to a statistically significant decrease in the microbial count of E. faecalis and P. aeruginosa along with an improved push-out bond strength of the root canal filling material with root.
研究常规根管治疗（cRCT）联合光动力疗法（aPDT）对感染的 C 形根管内微生物生物膜的疗效。
本体外报告中，将 20 颗新鲜离体下颌磨牙 C 形根管配置牙接种 E. faecalis 和 P. aeruginosa 以在预备好的根管系统中产生三天的生物膜。PDT 使用氯（ce6）和聚乙烯亚胺（PEI）的组合作为光敏剂（PS）。采用 200µ 光纤将 660nm 二极管激光输送到根管中，并将其与利用抗菌冲洗和机械清创的常规牙髓治疗进行比较和结合。
使用 aPDT（组 2）后，E. faecalis 和 P. aeruginosa 的数量从治疗前的 12.84±2.18 CFU/mL 显著减少至 5.13±0.67 CFU/mL，从 14.06±3.98 CFU/mL 减少至 4.82±1.05 CFU/mL。在两组研究样本中，治疗后均观察到两种微生物的细菌计数均有统计学意义的降低（p<0.05）。组 2（8.42±1.14 MPa）的试件显示出最高的平均推出粘结强度，而组 1 的试件则显示出最低的平均推出粘结强度（7.08±1.09 MPa）。ANOVA 显示研究组之间没有统计学差异（p=0.676）。独立 t 检验显示，研究组中颈段的平均推出粘结强度评分高于根尖和中段（p<0.05）。
在 C 形根管中，光动力疗法作为常规根管治疗的辅助手段的应用有助于统计显著降低 E. faecalis 和 P. aeruginosa 的微生物计数，并提高根管填充材料与根的推出粘结强度。
