Department of Conservative Dental Sciences, College of Dentistry, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia.
Department of Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.
Photodermatol Photoimmunol Photomed. 2022 Jul;38(4):328-333. doi: 10.1111/phpp.12751. Epub 2021 Nov 22.
BACKGROUND/PURPOSE: The aim was to assess the effectiveness of antimicrobial photodynamic therapy (aPDT) as an adjunct to mechanical instrumentation (MI) in reducing Enterococcus faecalis (E faecalis) and Candida albicans (C albicans) counts from C-shaped root-canals.
Teeth with C-shaped canals as identified on cone beam computed tomographic images were included. Following incubation with E faecalis and C albicans, samples were divided into 4 groups a four follows: Group-1: MI with adjunct aPDT; Group-2: aPDT alone; Group-3: MI alone; and Group-4: no treatment. The pulp chambers of these teeth were exposed and coronal pulp was mechanically derided using sterile endodontic excavators. Using flexible files, all canals were debrided up to size #30 K-files with intermittent irrigation with 2.5% sodium hypochlorite irrigation. In Groups 1 and 3, methylene-blue was injected in all canals and aPDT was performed using a 600 nm diode laser. Sample-size estimation was done on data from a pilot investigation and group-comparisons were done using one way analysis of variance and Bonferroni post-hoc adjustment tests. P < .05 was considered statistically significant.
Sixty periodontally hopeless mandibular second molars with C-shaped canals were included. There was a statistically significant reduction CFU/mL of E faecalis (P < .001) and C albicans (P < .001). At post-operative microbial assessments in Group-1. There was no difference CFU/mL of E faecalis and C albicans at post-operative microbial assessments in Groups 2-4.
MI with adjunct aPDT is more effective in reducing count of E faecalis and C albicans from C-shaped root canals.
背景/目的：本研究旨在评估抗菌光动力疗法（aPDT）作为机械器械（MI）辅助治疗在减少 C 形根管中粪肠球菌（E faecalis）和白色念珠菌（C albicans）数量的有效性。
纳入锥形束计算机断层扫描图像上显示为 C 形根管的牙齿。在孵育 E faecalis 和 C albicans 后，将样本分为以下 4 组：组 1：MI 联合 aPDT；组 2：单独 aPDT；组 3：单独 MI；组 4：无治疗。这些牙齿的牙髓腔被暴露出来，并用无菌根管锉将冠部牙髓机械切除。使用柔性锉，所有根管均用 2.5%次氯酸钠冲洗液进行间歇性冲洗，直至达到#30 K 文件大小。在组 1 和 3 中，将亚甲蓝注入所有根管，并使用 600nm 二极管激光进行 aPDT。样本量估计是基于初步研究的数据进行的，组间比较采用单因素方差分析和 Bonferroni 事后检验。P<.05 被认为具有统计学意义。
纳入了 60 颗牙周病无望的下颌第二磨牙，具有 C 形根管。E faecalis（P<0.001）和 C albicans（P<0.001）的 CFU/mL 均有统计学显著降低。在组 1 的术后微生物评估中，E faecalis 和 C albicans 的 CFU/mL 没有差异。在组 2-4 的术后微生物评估中。
MI 联合 aPDT 更有效地减少 C 形根管中粪肠球菌和白色念珠菌的数量。
