Advanced Centre for Tissue Engineering, Department of Biochemistry, University of Kerala, Thiruvananthapuram 695 581, India.
J Biosci. 2021;46.
Current treatment systems for tendon injuries are very few and do not ensure complete cure. This is a serious health concern for sports persons and the aged population. It is known that the nano- or microsized particles of natural products such as /cumin seed () has been used traditionally as a home remedy for the treatment of tendon injuries. Nevertheless, these particles are likely to perform better due to their smaller size, increased absorption and local delivery in conjunction with nanotechnology. In this context, the major objective of this study was to synthesize silver-capped nanoparticles using aqueous extract of (CCE) and to assess their non-cytotoxic effect with the perspective of clinical application to enhance tendon tissue regeneration. The presence of phytochemicals in CCE was studied by qualitative and quantitative methods. nanoparticles (CCNP) were synthesized by the bioreduction method using silver nitrate and the particles were characterized by X-ray diffraction analysis (XRD), Fourier Transform Infra Red Spectroscopy (FTIR), Zeta potential measurement and scanning electron microscopy (SEM). The antioxidant effect of the particles was studied using total antioxidant activity and reducing power assay. Simultaneously, primary Tenocytes were isolated from rabbit Achilles tendon by collagenase and dispase digestion/treatment and characterized for Type 1 collagen. Further, non-cytotoxicity of the CCNP in direct contact with L929 mouse fibroblast cells and primary Tenocytes, respectively, was evaluated by MTT assay. Physico-chemical characterizations confirmed the formation and stability of the nanosize of CCNP with antioxidant property. Again, MTT assay confirmed the non-cytotoxicity of CCNP with L929 fibroblasts and primary Tenocytes. CCNP may be attributed as an ideal candidate for therapeutic application towards a faster restoration of worn-out/injured tendon tissue confronted by the geriatric and athlete communities.
目前针对肌腱损伤的治疗方法非常有限，无法确保完全治愈。这是运动员和老年人群体面临的一个严重健康问题。众所周知，天然产物的纳米或微米级颗粒，如/孜然种子（），传统上一直被用作肌腱损伤治疗的家庭疗法。然而，由于这些颗粒尺寸更小，吸收增加，与纳米技术结合实现局部递送，因此它们可能会表现得更好。在这种情况下，本研究的主要目标是使用（CCE）的水提物合成银纳米颗粒，并评估其非细胞毒性作用，以期临床应用于增强肌腱组织再生。通过定性和定量方法研究 CCE 中植物化学物质的存在。使用硝酸银通过生物还原法合成 纳米颗粒（CCNP），并通过 X 射线衍射分析（XRD）、傅里叶变换红外光谱（FTIR）、Zeta 电位测量和扫描电子显微镜（SEM）对颗粒进行表征。使用总抗氧化活性和还原力测定法研究颗粒的抗氧化作用。同时，通过胶原酶和Dispase 消化/处理从兔跟腱中分离原代 Tenocytes，并对其进行 1 型胶原蛋白鉴定。此外，通过 MTT 测定法分别评估 CCNP 与 L929 小鼠成纤维细胞和原代 Tenocytes 直接接触时的非细胞毒性。物理化学特性证实了 CCNP 的纳米尺寸的形成和稳定性，具有抗氧化特性。再次，MTT 测定法证实 CCNP 对 L929 成纤维细胞和原代 Tenocytes 无细胞毒性。CCNP 可归因于一种理想的候选药物，可用于治疗因老龄化和运动员群体而磨损/受伤的肌腱组织的更快恢复。
