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脯氨酰羟化酶旁系同源物在底物特异性方面表现出高度相似性。

Prolyl Hydroxylase Paralogs in Show High Similarity With Regard to Substrate Specificity.

作者信息

Mócsai Réka, Göritzer Kathrin, Stenitzer David, Maresch Daniel, Strasser Richard, Altmann Friedrich

机构信息

Department of Chemistry, Institute of Biochemistry, University of Natural Resources and Life Sciences, Vienna, Austria.

Department of Applied Genetics and Cell Biology, Institute of Plant Biotechnology and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria.

出版信息

Front Plant Sci. 2021 Mar 2;12:636597. doi: 10.3389/fpls.2021.636597. eCollection 2021.

DOI:10.3389/fpls.2021.636597
PMID:33737944
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7960765/
Abstract

Plant glycoproteins display a characteristic type of -glycosylation where short arabinans or larger arabinogalactans are linked to hydroxyproline. The conversion of proline to 4-hydroxyproline is accomplished by prolyl-hydroxylases (P4Hs). Eleven putative P4Hs, which fall in four homology groups, have been identified by homology searches using known P4H sequences. One member of each of these groups has been expressed in insect cells using the baculovirus expression system and applied to synthetic peptides representing the -glycosylated region of erythropoietin (EPO), IgA1, Art v 1 and the glycoprotein STRUBBELIG. Unlike the situation in the moss , where one particular P4H was mainly responsible for the oxidation of erythropoietin, the tobacco P4Hs exhibited rather similar activities, albeit with biased substrate preferences and preferred sites of oxidation. From a biotechnological viewpoint, this result means that silencing/knockout of a single in cannot be expected to result in the abolishment of the plant-specific oxidation of prolyl residues in a recombinant protein.

摘要

植物糖蛋白呈现出一种独特的O-糖基化类型，其中短阿拉伯聚糖或较大的阿拉伯半乳聚糖与羟脯氨酸相连。脯氨酸向4-羟脯氨酸的转化是由脯氨酰羟化酶（P4Hs）完成的。通过使用已知的P4H序列进行同源性搜索，已鉴定出11种推定的P4Hs，它们分为四个同源组。这些组中的每个组的一个成员已使用杆状病毒表达系统在昆虫细胞中表达，并应用于代表促红细胞生成素（EPO）、IgA1、Art v 1和糖蛋白STRUBBELIG的O-糖基化区域的合成肽。与苔藓中的情况不同，在苔藓中一种特定的P4H主要负责促红细胞生成素的氧化，烟草P4Hs表现出相当相似的活性，尽管具有偏向的底物偏好和优先的氧化位点。从生物技术的角度来看，这一结果意味着不能期望在烟草中沉默/敲除单个P4H会导致重组蛋白中脯氨酰残基的植物特异性氧化被消除。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c82/7960765/48c0011cf65f/fpls-12-636597-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c82/7960765/73ad68e48dbd/fpls-12-636597-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c82/7960765/e423df62e99b/fpls-12-636597-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c82/7960765/d4c1dc4ce93a/fpls-12-636597-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c82/7960765/1e4abeda0248/fpls-12-636597-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c82/7960765/48c0011cf65f/fpls-12-636597-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c82/7960765/73ad68e48dbd/fpls-12-636597-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c82/7960765/e423df62e99b/fpls-12-636597-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c82/7960765/d4c1dc4ce93a/fpls-12-636597-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c82/7960765/1e4abeda0248/fpls-12-636597-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c82/7960765/48c0011cf65f/fpls-12-636597-g005.jpg

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